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Characterization and toxicity evaluation of air-borne particles released by grinding from two dental resin composites in vitro
Dental Materials ( IF 4.6 ) Pub Date : 2021-04-10 , DOI: 10.1016/j.dental.2021.03.011
L M A Camassa 1 , T K Ervik 1 , F D Zegeye 1 , I Mdala 2 , H Valen 3 , V Ansteinsson 4 , S Zienolddiny 1
Affiliation  

Objective

The project aims to evaluate whether inhalation of particles released upon grinding of dental composites may pose a health hazard to dentists. The main objective of the study was to characterize the dust from polymer-based dental composites ground with different grain sized burs and investigate particle uptake and the potential cytotoxic effects in human bronchial cells.

Methods

Polymerized blocks of two dental composites, Filtek™ Z250 and Filtek™ Z500 from 3M™ ESPE, were ground with super coarse (black) and fine (red) burs inside a glass chamber.

Ultrafine airborne dust concentration and particle size distribution was measured real-time during grinding with a scanning mobility particle sizer (SMPS). Filter-collected airborne particles were characterized with dynamic light scattering (DLS) and scanning electron microscopy (SEM).

Human bronchial epithelial cells (HBEC-3KT) were exposed to the dusts in dose-effect experiments. Toxicity was measured with lactate dehydrogenase (LDH) assay and cell counting kit-8 (CCK8). Cellular uptake was observed with transmission electron microscopy (TEM).

Results

Airborne ultrafine particles showed that most particles were in the size range 15−35 nm (SMPS). SEM analysis proved that more than 80% of the particles have a minimum Feret diameter less than 1 μm. In solution (DLS), the particles have larger diameters and tend to agglomerate. Cell toxicity (LDH, CCK8) is shown after 48 h and 72 h exposure times and at the highest doses. TEM showed presence of the particles within the cell cytoplasm.

Significance

Prolonged and frequent exposure through inhalation may have negative health implications for dentists.



中文翻译:

两种牙科树脂复合材料体外研磨释放的气载颗粒表征及毒性评价

客观的

该项目旨在评估吸入牙科复合材料研磨时释放的颗粒是否会对牙医造成健康危害。该研究的主要目的是表征用不同粒度的车针研磨的聚合物基牙科复合材料产生的粉尘,并研究颗粒吸收和人体支气管细胞的潜在细胞毒性作用。

方法

两种牙科复合材料的聚合块,来自 3M™ ESPE 的 Filtek™ Z250 和 Filtek™ Z500,在玻璃室内用超粗(黑色)和细(红色)车针研磨。

在研磨过程中使用扫描迁移性粒度仪 (SMPS) 实时测量超细气载粉尘浓度和粒度分布。用动态光散射 (DLS) 和扫描电子显微镜 (SEM) 对过滤器收集的空气中的颗粒进行表征。

在剂量效应实验中,人类支气管上皮细胞 (HBEC-3KT) 暴露于粉尘中。用乳酸脱氢酶 (LDH) 测定和细胞计数试剂盒 8 (CCK8) 测量毒性。用透射电子显微镜(TEM)观察细胞摄取。

结果

空气中的超细颗粒显示大多数颗粒的尺寸范围为 15-35 nm (SMPS)。SEM 分析证明超过 80% 的颗粒具有小于 1 μm 的最小 Feret 直径。在溶液 (DLS) 中,颗粒具有较大的直径并易于聚集。细胞毒性 (LDH, CCK8) 显示在 48 小时和 72 小时暴露时间和最高剂量后。TEM显示细胞质内存在颗粒。

意义

通过吸入长时间和频繁接触可能对牙医产生负面的健康影响。

更新日期:2021-06-13
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