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Antimicrobial properties of Lactobacillus cell‐free supernatants against multidrug‐resistant urogenital pathogens
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-04-09 , DOI: 10.1002/mbo3.1173 Marina Scillato 1 , Ambra Spitale 1 , Gino Mongelli 1 , Grete Francesca Privitera 1 , Katia Mangano 2 , Antonio Cianci 3 , Stefania Stefani 1 , Maria Santagati 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-04-09 , DOI: 10.1002/mbo3.1173 Marina Scillato 1 , Ambra Spitale 1 , Gino Mongelli 1 , Grete Francesca Privitera 1 , Katia Mangano 2 , Antonio Cianci 3 , Stefania Stefani 1 , Maria Santagati 1
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The healthy vaginal microbiota is dominated by Lactobacillus spp., which provide an important critical line of defense against pathogens, as well as giving beneficial effects to the host. We characterized L. gasseri 1A‐TV, L. fermentum 18A‐TV, and L. crispatus 35A‐TV, from the vaginal microbiota of healthy premenopausal women, for their potential probiotic activities. The antimicrobial effects of the 3 strains and their combination against clinical urogenital bacteria were evaluated together with the activities of their metabolites produced by cell‐free supernatants (CFSs). Their beneficial properties in terms of ability to interfere with vaginal pathogens (co‐aggregation, adhesion to HeLa cells, biofilm formation) and antimicrobial activity mediated by CFSs were assessed against multidrug urogenital pathogens (S. agalactiae, E. coli, KPC‐producing K. pneumoniae, S. aureus, E. faecium VRE, E. faecalis, P. aeruginosa, P. mirabilis, P. vulgaris, C. albicans, C. glabrata). The Lactobacilli tested exhibited an extraordinary ability to interfere and co‐aggregate with urogenital pathogens, except for Candida spp., as well as to adhere to HeLa cells and to produce biofilm in the Lactobacillus combination. Lactobacillus CFSs and their combination revealed a strong bactericidal effect on the multidrug resistant indicator strains tested, except for E. faecium and E. faecalis. The antimicrobial activity was maintained after heat treatment but decreased after enzymatic treatment. All Lactobacilli showed lactic dehydrogenase activity and production of D‐ and L‐lactic acid isomers on Lactobacillus CFSs, while only 1A‐TV and 35A‐TV released hydrogen peroxide and carried helveticin J and acidocin A bacteriocins. These results suggest that they can be employed as a new vaginal probiotic formulation and bio‐therapeutic preparation against urogenital infections. Further, in vivo studies are needed to evaluate human health benefits in clinical situations.
中文翻译：
乳酸杆菌无细胞上清液对多重耐药泌尿生殖道病原体的抗菌特性
健康的阴道微生物群由乳杆菌属主导，它提供了重要的防御病原体的关键防线，并为宿主提供了有益的影响。我们描述了L。加塞里1A-TV, L . 发酵18A-TV，和大号。卷曲的35A-TV，来自健康绝经前妇女的阴道微生物群，因为它们具有潜在的益生菌活性。评估了这 3 种菌株及其组合对临床泌尿生殖系统细菌的抗菌作用，以及由无细胞上清液 (CFS) 产生的代谢物的活性。它们在与阴道病原体（共聚集，粘附到HeLa细胞，生物膜的形成）和抗微生物活性通过氯氟烃介导的干扰的能力方面是有益的性能进行了对多药泌尿生殖病原体（评估小号。无乳链球菌，ê。大肠杆菌，产KPC ķ。肺炎，小号，金黄色葡萄球菌，乙。粪便VRE, E . 粪，P。铜绿假单胞菌，P。奇异变形杆菌，P。普通的，C . 白色念珠菌，C。光滑的）。所测试的乳杆菌表现出非凡的能力，可以干扰和共聚集泌尿生殖道病原体（念珠菌属除外），以及粘附在 HeLa 细胞上并在乳杆菌组合中产生生物膜。乳酸菌氯氟烃和它们的组合显示上测试，除了多药耐药性菌株指示器很强的杀菌作用ë。粪便和大肠杆菌。粪便。热处理后抗菌活性保持不变，酶处理后抗菌活性下降。所有乳杆菌表现出乳酸脱氢酶活性和生产D-和L-乳酸异构体的乳酸杆菌氯氟烃，而只有图1A-TV和35A-TV释放过氧化氢和进行helveticin J和acidocin一种细菌素。这些结果表明它们可以用作一种新的阴道益生菌制剂和生物治疗制剂来对抗泌尿生殖系统感染。此外，需要体内研究来评估临床情况下的人类健康益处。
更新日期：2021-04-09
中文翻译：
乳酸杆菌无细胞上清液对多重耐药泌尿生殖道病原体的抗菌特性
健康的阴道微生物群由乳杆菌属主导，它提供了重要的防御病原体的关键防线，并为宿主提供了有益的影响。我们描述了L。加塞里1A-TV, L . 发酵18A-TV，和大号。卷曲的35A-TV，来自健康绝经前妇女的阴道微生物群，因为它们具有潜在的益生菌活性。评估了这 3 种菌株及其组合对临床泌尿生殖系统细菌的抗菌作用，以及由无细胞上清液 (CFS) 产生的代谢物的活性。它们在与阴道病原体（共聚集，粘附到HeLa细胞，生物膜的形成）和抗微生物活性通过氯氟烃介导的干扰的能力方面是有益的性能进行了对多药泌尿生殖病原体（评估小号。无乳链球菌，ê。大肠杆菌，产KPC ķ。肺炎，小号，金黄色葡萄球菌，乙。粪便VRE, E . 粪，P。铜绿假单胞菌，P。奇异变形杆菌，P。普通的，C . 白色念珠菌，C。光滑的）。所测试的乳杆菌表现出非凡的能力，可以干扰和共聚集泌尿生殖道病原体（念珠菌属除外），以及粘附在 HeLa 细胞上并在乳杆菌组合中产生生物膜。乳酸菌氯氟烃和它们的组合显示上测试，除了多药耐药性菌株指示器很强的杀菌作用ë。粪便和大肠杆菌。粪便。热处理后抗菌活性保持不变，酶处理后抗菌活性下降。所有乳杆菌表现出乳酸脱氢酶活性和生产D-和L-乳酸异构体的乳酸杆菌氯氟烃，而只有图1A-TV和35A-TV释放过氧化氢和进行helveticin J和acidocin一种细菌素。这些结果表明它们可以用作一种新的阴道益生菌制剂和生物治疗制剂来对抗泌尿生殖系统感染。此外，需要体内研究来评估临床情况下的人类健康益处。
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