Diabetes is considered one of the most important health emergencies worldwide and Egypt has 8.2 million diabetic patients according to the International Diabetes Federation report in 2017. The objective of this study was to monitor the time-course variation in the metabolic profile of diabetic rats to detect urinary metabolic biomarkers using the metabolomics approach. Type 2 diabetes was induced in male Wistar albino rats using a single intraperitoneal injection of 40 mg/kg of streptozotocin following oral administration of 10% fructose in drinking water for 3 weeks. Then, urine was collected for 24 h from rats at three time points (0, 2, and 4 weeks after confirmation of diabetes), and were analyzed by nuclear magnetic resonance (H¹-NMR), followed by multivariate data analysis. The results from H¹-NMR pointed out that d-glucose, taurine, l-carnitine, l-fucose, 1,5-anhydrosorbitol, and d-galactose levels showed consistent significant variation (p < 0.05) between the positive (diabetic) and negative (normal) controls during the whole experimental period. Also, with the disease progression, myoinositol, and l-phenylalanine levels were significantly altered (p < 0.05) after 2 weeks and this alteration was maintained till the end of the 4-week experimental period in the positive control group. From the results of the present study, it could be concluded that we cannot depend only on glucose levels for prognostic purposes since there are other metabolic disturbances in diabetes which need to be tracked for better disease prognosis.

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基于时间的 2 型糖尿病尿代谢标志物调查：糖尿病管理的代谢组学方法

根据国际糖尿病联盟 2017 年的报告，糖尿病被认为是全球最重要的突发卫生事件之一，埃及有 820 万糖尿病患者。本研究的目的是监测糖尿病大鼠代谢特征的时程变化，以检测使用代谢组学方法的尿代谢生物标志物。在口服 10% 果糖饮用水 3 周后，使用单次腹腔注射 40 mg/kg 链脲佐菌素在雄性 Wistar 白化病大鼠中诱发 2 型糖尿病。然后，在三个时间点（确认糖尿病后0、2和4周）从大鼠中收集24小时的尿液，并通过核磁共振（H ¹ -NMR）进行分析，然后进行多变量数据分析。来自 H 的结果¹ H-NMR指出d葡萄糖，牛磺酸，升肉碱，升-岩藻糖，1,5-脱水山梨醇，和d半乳糖水平显示出一致的显著变化（p 的正（糖尿病）和负极之间<0.05）（正常) 在整个实验期间进行控制。此外，随着疾病进展，肌醇和l-苯丙氨酸水平显着改变（p < 0.05) 2 周后，这种变化在阳性对照组中一直保持到 4 周实验期结束。从本研究的结果可以得出结论，我们不能仅仅依靠葡萄糖水平来预测预后，因为糖尿病中还有其他代谢紊乱需要跟踪以获得更好的疾病预后。