Abstract

The resistance to calcification of bovine jugular veins (BJVs) coated by chitosan or bisphosphonate deposited from solutions in water/subcritical carbon dioxide (carbonic acid) system was studied for the first time. Samples of veins subjected to hybrid decellularization in supercritical CO₂ and coated by chitosan and bisphosphonate deposited from solutions in carbonic acid at elevated pressure (P = 30 MPa) and at room temperature were studied. The calcification of the treated veins, especially bisphosphonate-coated ones, was found to decrease by Δ = 90 ± 5%, p < 0.05, in comparison with the generally accepted gold standard (BJVs cross-linked with glutaraldehyde and decellularized with sodium dodecyl sulfate). It was found that significant suppression of BJV calcification requires a combination of properties of the coating, namely, the ability to cover free aldehyde groups and the ability to suppress the growth of pathogenic microflora (antimicrobial activity).

中文翻译：

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碳酸溶液中沉积的涂层对牛颈静脉钙化的抵抗性研究

摘要

首次研究了从水/亚临界二氧化碳（碳酸）溶液中沉积的壳聚糖或双膦酸酯包被的牛颈静脉钙化的抵抗力。研究了在超临界CO _2中经历混合脱细胞并在室温（P = 30 MPa）下从碳酸溶液中沉积的壳聚糖和双膦酸酯包被的静脉样品。发现处理过的静脉，特别是双膦酸盐处理过的静脉的钙化降低了Δ= 90±5％，p与普遍接受的金标准（BJV与戊二醛交联并由十二烷基硫酸钠脱细胞的BJV）相比，其<0.05。已经发现，对BJV钙化的显着抑制需要涂层的性能的组合，即覆盖游离醛基的能力和抑制病原性微生物群生长的能力（抗微生物活性）。