Wild Chi-Nan agarwood is regarded as the highest quality agarwood from Aquilaria spp. However, the comprehensive research on chemical composition of wild Chi-Nan agarwood is limited. An integrated strategy using SHS-GC-MS and UPLC-Q/Tof-MS was applied to explore the phytochemical characteristics of a kind of agarwood induced from a newly identified germplasm of Chi-Nan A. sinensis. Progenesis QI and MS-Dial were used to preprocess the UPLC-Q/Tof-MS and GC-MS raw data, respectively. Principle component analysis (PCA) and orthogonal partial least squares to latent structure-discriminant analysis (OPLS-DA) models were built to discriminate Chi-Nan agarwood from ordinary agarwood and to screen potential distinguishing components between them. In this study, we clarified the distinguishing differences between Chi-Nan agarwood and ordinary agarwood. The difference is mainly manifested in the average contents of 2-(2-phenylethyl)chromone and 2-[2-(4′-methoxybenzene)ethyl]chromone, which are 170 and 420 times higher in Chi-Nan agarwood than in ordinary agarwood, respectively, while the contents of 5,6,7,8-diepoxy-2-(2-phenylethyl)chromones(DEPECs), 5,6-epoxy-2-(2-phenylethyl)chromones(EPECs), and 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones(THPECs) such as agarotetrol are extremely low. The content of the main sesquiterpenes in Chi-Nan agarwood was higher than that in ordinary agarwood, especially in regard to guaiane and eudesmane derivatives. In addition, there were significant differences in the contents of low-molecular-weight aromatic compounds such as 2-methyl-4H-1-benzopyran-4-one, 4-methoxybenzaldehyde, and 2-hydroxybenzaldehyde between Chi-Nan agarwood and ordinary agarwood. All the mentioned main chemical characteristics of this new Chi-Nan agarwood were coincident with those of the rare wild Chi-Nan agarwood from A. malaccensis, A. sinensis, and A. crassna. We reported differences in 2-(2-phenylethyl)chromones, sesquiterpenes, and low-molecular-weight aromatic compounds between Chi-Nan agarwood and ordinary agarwood from A. sinensis for the first time; it is necessary to evaluate the agarwood from the new-found Chi-Nan germplasm.

中文翻译：

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新发现的沉香池南种质诱导的池南沉香与普通沉香相比具有显着的植物化学特性


野生赤南沉香被认为是沉香属中最优质的沉香。然而，对野生池南沉香化学成分的综合研究还很有限。采用 SHS-GC-MS 和 UPLC-Q/Tof-MS 的综合策略来探索由新鉴定的池南沉香种质诱导的沉香的植物化学特性。 Progenesis QI 和 MS-Dial 分别用于预处理 UPLC-Q/Tof-MS 和 GC-MS 原始数据。建立主成分分析（PCA）和正交偏最小二乘潜在结构判别分析（OPLS-DA）模型来区分池南沉香和普通沉香，并筛选它们之间潜在的区分成分。在本研究中，我们阐明了池南沉香与普通沉香之间的区别。差异主要表现在2-(2-苯乙基)色酮和2-[2-(4′-甲氧基苯)乙基]色酮的平均含量，池南沉香分别比普通沉香高170倍和420倍。分别为 5,6,7,8-二环氧-2-(2-苯乙基)色酮 (DEPECs)、5,6-环氧-2-(2-苯乙基)色酮 (EPECs) 和 5,琼脂四醇等 6,7,8-四氢-2-(2-苯乙基)色酮 (THPEC) 含量极低。池南沉香中主要倍半萜类化合物的含量高于普通沉香，尤其是愈创木烷和桉树烷衍生物。此外，赤南沉香与普通沉香的2-甲基-4H-1-苯并吡喃-4-酮、4-甲氧基苯甲醛、2-羟基苯甲醛等低分子量芳香族化合物的含量存在显着差异。 。 这种新的池南沉香的所有上述主要化学特性均与珍贵的野生池南沉香（ A. malaccensis 、 A. sinensis和A. crassna）一致。首次报道了池南沉香与普通沉香中2-(2-苯乙基)色酮、倍半萜烯和低分子芳香族化合物的差异；有必要对新发现的池南种质沉香进行评价。