Interleukin-12 (IL-12) is a heterodimeric cytokine encoded by two separate genes, IL12A and IL12B, which may play a regulatory role in allergen-induced inflammation through CD4⁺ T-cell subsets polarization. The aim of this study was to investigate the association of single-nucleotide polymorphisms (SNPs) in the IL12B gene with susceptibility to allergic rhinitis (AR). We performed a case–control study including 130 AR patients and 130 healthy controls to evaluate the possible association between IL12B gene SNPs (rs3212227, rs6887695) and the risk of AR using the polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) method. Our results showed no significant association between IL12B rs3212227 A > C polymorphism with AR. In contrast, the GC genotype of rs6887695 G > C was associated with susceptibility to AR in comparison with the GG genotype (p = 0.049, OR = 1.684, 95% CI: 1.002–2.83). We also observed a statistically significant difference in the additive model (GC versus GG + CC, p = 0.03, OR = 1.705, 95% CI: 1.040–2.794) for SNPs rs6887695. Furthermore, haplotypes analysis demonstrated that C–C haplotype was associated with an increased risk of AR (p = 0.01, OR = 1.845, 95% CI: 1.114–3.057). Our findings suggest that IL12B rs6887695 polymorphism may be a potential biomarker for susceptibility to AR in an Iranian population.

^{白细胞介素12（IL-12）是一种异源二聚体细胞因子，由两个独立的基因IL12A和IL12B编码，可能通过CD4 +}在过敏原诱导的炎症中发挥调节作用T 细胞亚群极化。本研究的目的是研究 IL12B 基因中的单核苷酸多态性 (SNP) 与过敏性鼻炎 (AR) 易感性的关系。我们进行了一项病例对照研究，包括 130 名 AR 患者和 130 名健康对照，以使用聚合酶链反应-限制性片段长度多态性 (PCR-RFLP) 方法评估 IL12B 基因 SNP（rs3212227、rs6887695）与 AR 风险之间的可能关联. 我们的结果显示 IL12B rs3212227 A > C 多态性与 AR 之间没有显着关联。相比之下，与 GG 基因型相比，rs6887695 G > C 的 GC 基因型与 AR 的易感性相关（p = 0.049，OR = 1.684，95% CI：1.002–2.83）。我们还观察到 SNP rs6887695 的加性模型（GC 与 GG + CC，p  = 0.03，OR = 1.705，95% CI：1.040-2.794）存在统计学上的显着差异。此外，单倍型分析表明，C-C 单倍型与 AR 风险增加相关（p  = 0.01，OR = 1.845，95% CI：1.114-3.057）。我们的研究结果表明，IL12B rs6887695 多态性可能是伊朗人群对 AR 易感性的潜在生物标志物。