Bacillus anthracis, the causative agent of anthrax is a Gram-positive, non-motile, spore forming bacterium. Its spores can persist in soil and water for years and can also be aerosolized. A rapid, sensitive and specific method to detect B. anthracis is important for clinical management and preventing spread of anthrax. Loop-mediated isothermal amplification (LAMP) assay is a rapid technique that amplifies target DNA in isothermal conditions with high sensitivity and specificity. In this study, a LAMP assay set targeting a chromosomal and two plasmid markers was developed. The individual assays of the LAMP set targeting pXO1 plasmid (lef), pXO2 plasmid (capB), and chromosome (BA5345) sequences could detect 10, 250, and 100 fg of genomic DNA and 10, 100, and 50 copies of the DNA targets harboured in recombinant plasmids, respectively. The lef and capB LAMP assays could detect ≥ 1 × 10³ CFU per mL of bacteria in spiked human blood samples, while BA5345 LAMP assay could detect ≥ 1 × 10⁴ CFU of bacteria per mL of spiked blood. The amplification was monitored in real-time by turbidimeter, and visual detection was also accomplished under normal and UV light after adding SYBR Green 1 dye on completion of the reaction. The assay set was found to be highly sensitive and did not cross-react with the closely related Bacillus spp. and other bacterial strains used in the study.

炭疽芽孢杆菌是炭疽病的病原体，是一种革兰氏阳性、非运动性的孢子形成细菌。它的孢子可以在土壤和水中存留多年，也可以雾化。一种检测炭疽芽孢杆菌的快速、灵敏和特异的方法对于临床管理和预防炭疽传播非常重要。环介导等温扩增 (LAMP) 分析是一种快速技术，可在等温条件下以高灵敏度和特异性扩增目标 DNA。在这项研究中，开发了一个针对染色体和两个质粒标记的 LAMP 检测集。针对 pXO1 质粒 ( lef )、pXO2 质粒 ( capB ) 和染色体 ( BA5345) 序列可以分别检测 10、250 和 100 fg 的基因组 DNA 以及 10、100 和 50 份包含在重组质粒中的 DNA 靶标。所述LEF和CAPB LAMP测定可以检测≥1×10 ³  CFU每尖刺人体血液样品中的细菌中的溶液，同时BA5345 LAMP方法可以检测≥1×10 ⁴  ％的掺杂的血液毫升细菌的CFU。扩增通过浊度计实时监测，反应完成后加入SYBR Green 1染料后，在常光和紫外光下进行目视检测。发现该测定集高度敏感，并且不与密切相关的芽孢杆菌发生交叉反应属 和研究中使用的其他细菌菌株。