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Molecular characterization of marine and coastal fishes of Bangladesh through DNA barcodes
Ecology and Evolution ( IF 2.3 ) Pub Date : 2021-04-07 , DOI: 10.1002/ece3.7355 Md Sagir Ahmed 1 , Sujan Kumar Datta 2 , Tonmoy Saha 2 , Zarif Hossain 3
Ecology and Evolution ( IF 2.3 ) Pub Date : 2021-04-07 , DOI: 10.1002/ece3.7355 Md Sagir Ahmed 1 , Sujan Kumar Datta 2 , Tonmoy Saha 2 , Zarif Hossain 3
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This study describes the molecular characterization of marine and coastal fishes of Bangladesh based on the mitochondrial cytochrome c oxidase subunit I (COI) gene as a marker. A total of 376 mitochondrial COI barcode sequences were obtained from 185 species belonging to 146 genera, 74 families, 21 orders, and two classes of fishes. The mean length of the sequences was 652 base pairs. In Elasmobranchii (Sharks and rays), the average Kimura two parameter (K2P) distances within species, genera, families, and orders were 1.20%, 6.07%, 11.08%, and 14.68%, respectively, and for Actinopterygii, the average K2P distances within species, genera, families, and orders were 0.40%, 6.36%, 14.10%, and 24.07%, respectively. The mean interspecies distance was 16‐fold higher than the mean intraspecies distance. The K2P neighbor‐joining (NJ) trees based on the sequences generally clustered species in accordance with their taxonomic position. A total of 21 species were newly recorded in Bangladesh. High efficiency and fidelity in species identification and discrimination were demonstrated in the present study by DNA barcoding, and we conclude that COI sequencing can be used as an authentic identification marker for Bangladesh marine fish species.
中文翻译：
通过 DNA 条形码对孟加拉国海洋和沿海鱼类进行分子表征
本研究以线粒体细胞色素 c 氧化酶亚基 I (COI) 基因为标记，描述了孟加拉国海洋和沿海鱼类的分子特征。共获得鱼类21目、146属、74科、185种的线粒体COI条形码序列376条。序列的平均长度为 652 个碱基对。在板鳃亚纲（鲨鱼和鳐鱼）中，种、属、科和目内的平均木村二参数 (K2P) 距离分别为 1.20%、6.07%、11.08% 和 14.68%，而对于放线鳍鱼，平均 K2P 距离分别为 1.20%、6.07%、11.08% 和 14.68%。种、属、科、目内的比例分别为0.40%、6.36%、14.10%、24.07%。平均种间距离比平均种内距离高 16 倍。基于序列的 K2P 邻接（NJ）树通常根据物种的分类位置对物种进行聚类。孟加拉国共新记录了21种。本研究通过 DNA 条形码证明了物种识别和区分的高效率和保真度，我们得出结论，COI 测序可以用作孟加拉国海洋鱼类物种的真实识别标记。
更新日期：2021-05-04
中文翻译：
通过 DNA 条形码对孟加拉国海洋和沿海鱼类进行分子表征
本研究以线粒体细胞色素 c 氧化酶亚基 I (COI) 基因为标记，描述了孟加拉国海洋和沿海鱼类的分子特征。共获得鱼类21目、146属、74科、185种的线粒体COI条形码序列376条。序列的平均长度为 652 个碱基对。在板鳃亚纲（鲨鱼和鳐鱼）中，种、属、科和目内的平均木村二参数 (K2P) 距离分别为 1.20%、6.07%、11.08% 和 14.68%，而对于放线鳍鱼，平均 K2P 距离分别为 1.20%、6.07%、11.08% 和 14.68%。种、属、科、目内的比例分别为0.40%、6.36%、14.10%、24.07%。平均种间距离比平均种内距离高 16 倍。基于序列的 K2P 邻接（NJ）树通常根据物种的分类位置对物种进行聚类。孟加拉国共新记录了21种。本研究通过 DNA 条形码证明了物种识别和区分的高效率和保真度，我们得出结论，COI 测序可以用作孟加拉国海洋鱼类物种的真实识别标记。
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