Penaeus vannamei is one of the commercially important aquaculture species in the world but sensitive to Vibrio anguillarum infection. To understand the immune response of P. vannamei against V. anguillarum infection, the transcriptomes of hepatopancreas and gills of P. vannamei were analysed before and after V. anguillarum infection. Total 6213 differentially expressed genes (DEGs) were obtained from the hepatopancreas, and 3780 were obtained from the gills. The DEGs of interest were screened to construct a heat map. The reliability and accuracy of the transcriptome data were verified using RT-qPCR. HSP70 and ARF6 were found highly expressed in both hepatopancreas and gills, while MLC2s encoded by two different gene sequences were found to be expressed in hepatopancreas and gills. Six immune-related DEGs were identified from the protein–protein interaction (PPI) network including the HSP70, ARF6 and MLC2s. The sub-network of PPI was constructed to explore the mode of interaction of immune-related DEGs with their neighbouring proteins. Based on the result of PPI network, we infer that phosphorylated MLC2-1 conducts signal transduction to induce leukocyte transendothelial migration in P. vannamei gills tissue while MLC2-2 plays a role in immunity of V. anguillarum infection not only through activating signal transduction to mediate immune responses but also through the formation of phagosomes in hepatopancreas tissue. HSP70 is associated with the transporting exogenous cargos through presenting antigenic peptides in the process of V. anguillarum infection in P. vannamei. ARF6 interacts with Rab proteins to regulate phagolysosome formation by regulating GTPase activity in P. vannamei gills tissue. In hepatopancreas tissue, ARF6 plays a role in immunity by interacting with cytohesin. This study will contribute to further exploring the immune mechanism of P. vannamei to V. anguillarum infection.

中文翻译：

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转录组分析揭示了鳗弧菌感染后南美白对虾肝胰腺和鳃中免疫相关差异表达基因

南美白对虾是世界上具有重要商业价值的水产养殖品种之一，但敏感的弧菌 鳗感染。了解P的免疫反应。 南美白对虾对V。 鳗弧菌感染，肝胰脏的转录和鳃P。 在V之前和之后对南美白对虾进行了分析。 鳗鲡感染。从肝胰腺中获得了总共6213个差异表达基因（DEG），从鳃中获得了3780个。筛选感兴趣的 DEG 以构建热图。使用 RT-qPCR 验证了转录组数据的可靠性和准确性。HSP70 和 ARF6 在肝胰腺和鳃中均高度表达，而由两种不同基因序列编码的 MLC2 在肝胰腺和鳃中均有表达。从蛋白质 - 蛋白质相互作用（PPI）网络中鉴定出六个免疫相关的DEG，包括HSP70、ARF6和MLC2。构建了 PPI 的子网络，以探索免疫相关 DEG 与其相邻蛋白质的相互作用模式。基于PPI网络的结果，P。 南美白对虾鳃组织，而 MLC2-2 在V 的免疫中起作用。 鳗鲡感染不仅通过激活信号转导来介导免疫反应，而且还通过在肝胰腺组织中形成吞噬体。HSP70 通过在V过程中呈递抗原肽与运输外源货物有关。 P 中的鳗鲡感染。 南美白对虾。ARF6 与 Rab 蛋白相互作用，通过调节P 中的GTPase 活性来调节吞噬溶酶体的形成。 南美白对虾鳃组织。在肝胰腺组织中，ARF6 通过与细胞粘附素相互作用而发挥免疫作用。本研究将有助于进一步探索P的免疫机制。 南美白对虾对V。 鳗鲡感染。