This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and possess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L⁻¹ and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54–2.30 mM L⁻¹). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide.

进行这项研究的目的是通过接种种子来改善苜蓿种子在压力条件下的发芽，铬浓度升高或镰刀菌感染。根据其抑制植物病原体和耐受增加的铬浓度的能力，选择了新的假芽孢杆菌BM1菌株作为孕育剂。根据16S rDNA序列从紫花苜蓿根际土壤中分离和鉴定了根际细菌。此外，在这项研究中，研究了菌株BM1产生植物激素吲哚-3-乙酸，铁载体，氰化氢，细胞外脂肪酶，纤维素酶，果胶酶和溶解磷酸盐的能力。BM1菌株在体外显示F. graminearum，F. proliferatum和F. oxysporum f。对真菌生长的强烈抑制率分别为59.68％，48.72％和43.59％。sp。medicaginis分别。据我们所知，这是首次报道假芽孢杆菌具有抗真菌潜力，并具有丰霉素和枯草杆菌蛋白酶的生物合成基因。这种根瘤菌可产生吲哚-3-乙酸，铁载体，细胞外脂肪酶，纤维素和果胶酶。F. oxysporum f。侵染紫花苜蓿幼苗的抑制率。sp。药物（体外）从27.87％降至2.46％。施用BM1培养接种物达到了根和茎长的最高增加，分别为12.36％和21.43％。铬浓度为0.11 mM L ^-1的情况下，接种BM1的种子与对照相比没有抑制作用，与对照样品相比（1.54–2.30 mM L ^-1），当铬浓度增加时，抑制作用降低。新菌株BM1可能是对污染土壤进行植物修复的好选择，同时也是生物肥料或生物杀真菌剂中的良好活性剂。