当前位置: X-MOL 学术Flora › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Pollination related temporal sequences of reproductive development and tracing the path of pollen tubes in Cymbidium pendulum (Roxb.) Sw., an ornamental orchid
Flora ( IF 1.7 ) Pub Date : 2021-04-08 , DOI: 10.1016/j.flora.2021.151813
Lucky K Attri , Harsh Nayyar

Orchids, known for their exquisite beauty, are highly priced as cut flowers and potted plants at national and International market. More than I lac manmade hybrids are produced every year at intra to pleurigeneric level. Therefore, it is of imperative interest to unravel the all aspects of orchids in details. In the present study, the flowers of Cymbidium pendulum, an ornamental important orchids, have been investigated. It is observed that the flowers of C. pendulum started wilting in 20 days without pollination and senesced within 8 days after the act of pollination, suggesting a rapid progression of senescence related events triggered by pollination. The placenta became meristematically active only after pollination. The ovule primordium consisted of an axial filament 5–7 cells high enclosed by epidermal cells. The uppermost cell of the nucellar filament differentiated into an archesporial cell at 20–26 days after pollination (DAP), which turned into megaspore mother cell at 26–30 DAP. The megaspore mother cell underwent meiosis resulting in the formation of a triad which converted in to an eight-nucleate polygonum type of embryo sac. Thus the total time taken to reach the organized embryo sac is between 55-60 days. The results were well confirmed by study with fluorescent microscope using fluorescent dye where the pollen tubes were observed to move through base of the column (6–10 DAP) and entered the ovarian locule (8–16 DAP) and grew on either side of the each placenta, stayed there till the embryo matures and then liberated male gametes for fertilization (54–58DAP). The fertilization occurred around 58–60 DAP. In the present studies, the detailed elucidation of post pollination reproductive development, along-with temporal sequences of path of pollen tubes, have generated valuable information, advantageously useful both for academicians and breeders.



中文翻译:

生殖发育和跟踪花粉管的路径中的授粉相关时间序列兰摆(的罗)西南。,观赏兰花

兰花以其精美的美而闻名,在国内和国际市场上都以高价出售切花和盆栽植物。每年,在内部到胸膜的水平上,人工杂交的杂种的产量都超过了Ilac。因此,必须详细了解兰花的各个方面,这是当务之急。在本研究中,已对兰花大花ym兰(一种重要的观赏兰花)的花朵进行了研究。观察到C.摆的花朵在没有授粉的20天内开始枯萎,并在授粉后8天内衰老,这表明由授粉引发的衰老相关事件迅速发展。仅在授粉后胎盘才有分生组织活性。胚珠原基由表皮细胞包围的5-7个轴向丝状细胞组成。授粉(DAP)后20–26天,细胞核细丝的最上层细胞分化为原型孢子细胞,而DAP在26–30天后变为大孢子母细胞。大孢子母细胞经历减数分裂,导致三联体的形成,该三联体转化为八核polygon精类型的胚囊。因此,到达有组织的胚囊所需的总时间在55至60天之间。使用荧光染料的荧光显微镜研究很好地证实了结果,在该显微镜下观察到花粉管穿过柱底(6-10 DAP)进入卵巢房(8-16 DAP)并在卵囊的任一侧生长。每个胎盘都待在那里,直到胚胎成熟,然后释放雄配子进行受精(54–58DAP)。受精发生在58-60 DAP左右。在当前的研究中,对授粉后生殖发育的详细阐明以及花粉管的路径时间序列已产生了有价值的信息,有利于院士和育种者。呆在那里直到胚胎成熟,然后释放雄配子进行受精(54-58DAP)。受精发生在58-60 DAP左右。在当前的研究中,对授粉后生殖发育的详细阐明以及花粉管的路径时间序列已产生了有价值的信息,有利于院士和育种者。呆在那里直到胚胎成熟,然后释放雄配子进行受精(54-58DAP)。受精发生在58-60 DAP左右。在当前的研究中,对授粉后生殖发育的详细阐明以及花粉管的路径时间序列已产生了有价值的信息,有利于院士和育种者。

更新日期:2021-04-16
down
wechat
bug