Considering the rapid transmission of the dengue virus, substantial efforts need to be conducted to ward-off the epidemics of dengue viruses. The control effort is depending on chemical insecticides and had aroused undesirable conflicts of insecticide resistance. Here, we study the entomopathogenic fungus, Metarhizium anisopliae as a promising new biological control agent for vector control. The pathogenicity effects of Metarhizium anisopliae against field and laboratory strains of Aedes albopictus and Aedes aegypti larvae were tested using the larvicidal bioassay technique. The results demonstrate that the treatments using M. anisopliae isolate MET-GRA4 were highly effective and able to kill 100% of both Ae. albopictus and Ae. aegypti mosquito larvae at a conidia concentration of 1 × 10⁶/ml within 7 days of the treatment period. The fungus displayed high larvicidal activity against laboratory and field strain of Ae. aegypti larvae with LC₅₀ values (9.6 × 10³/ml, 1.3 × 10³/ml) and LC₉₅ values (1.2 × 10⁶/ml, 5.5 × 10⁵/ml) respectively. For Ae. albopictus, LC₅₀ values for laboratory and field strains were (1.7 × 10⁴/ml, 2.7 × 10⁴/ml) and the LC₉₅ values were (2.1 × 10⁶/ml, 7.0 × 10⁵/ml) respectively. Interestingly, the susceptibility of field strain towards M. anisopliae was higher as compared to the laboratory strain Aedes larvae. In which, the causative agents of all the dead larvae were verified by the virulence of M. anisopliae and caused morphological deformities on larval body. The findings from this study identify this isolate could be an effective potential biocontrol agent for vector mosquitoes in Malaysia.

考虑到登革热病毒的快速传播，需要做出巨大努力来抵御登革热病毒的流行。防治工作取决于化学杀虫剂，并引起了不希望有的杀虫剂抗性冲突。在这里，我们研究昆虫病原真菌，Metarhizium anisopliae，作为一种有前途的新的生物媒介控制载体。使用杀幼虫生物测定技术，测试了Metanhizium anisopliae对白纹伊蚊和埃及伊蚊幼虫的田间和实验室菌株的致病作用。结果表明，使用M. anisopliae分离株MET-GRA4进行的治疗非常有效，并且能够杀死两者e albopictus和Ae。在治疗期间的7天内，分生孢子浓度为1×10 6 / ml的埃及埃及蚊幼虫。该真菌对Ae的实验室和野外菌株表现出很高的杀幼虫活性。埃及伊蚊幼虫的LC ₅₀值分别为（9.6×10 ³ / ml，1.3×10 ³ / ml）和LC ₉₅值（1.2×10 6 / ml，5.5×10 ⁵ / ml）。对于爱 纹伊蚊，LC _50个为实验室和现场菌株值分别为（1.7×10 ⁴ / ml的，2.7×10 ⁴ / ml）和所述LC _95个值分别为（2.1×10⁶/毫升，7.0×10 ⁵/ ml）。有趣的是，与实验室菌株伊蚊幼虫相比，田间菌株对分离的分枝杆菌的敏感性更高。其中，所有死幼虫的致病菌均已通过沙门氏菌的致病力进行了验证，并引起了幼虫体的形态畸变。这项研究的发现表明，这种分离株可能是马来西亚媒介蚊的有效潜在生物防治剂。