Microsatellite markers have been used extensively in studies of sperm competition and the genetic diversity of organisms. In the present study, we performed transcriptome sequencing for Velarifictorus aspersus and developed simple sequence repeat markers to identify valuable markers for genetic studies. The average total length of the V. aspersus transcriptome sequence was 7.70 Gb and it contained 7878 microsatellite loci. Sixty-four microsatellite loci were randomly selected for primer design and 39 loci were successfully amplified, among which 23 loci were polymorphic. The microsatellites were also tested in six other species from the Gryllidae family. Among the species belonging to the genus Velarifictorus, 22 loci were amplified in Velarifictorus micado, eight loci in Velarifictorus ornatus, and 3–6 loci in the other four cricket species.

微卫星标记已广泛用于精子竞争和生物遗传多样性的研究。在本研究中，我们进行了曲霉菌的转录组测序，并开发了简单的序列重复标记，以鉴定遗传学有价值的标记。V. aspersus转录组序列的平均总长度为7.70 Gb，包含7878个微卫星基因座。随机选择64个微卫星基因座进行引物设计，成功扩增39个基因座，其中23个基因座为多态性。还从Gryllidae家族的其他六个物种中测试了微卫星。在属于Velarifictorus属的物种中，22个位点被扩增。拟南芥（Vararifictorus micado），黑ar（Vararifictorus ornatus）中有8个基因座，其他4种板球物种中有3–6个基因座。