In the present study we performed a transcriptional analysis in order to evaluate changes in gene expression induced by exploration in prolonged times. The analysis was carried out 3, 10 and 20 days after exploration. We analyzed the modulation of the expression levels of Pfn2, Casp3, Pdrg1, Pea15, Ywhaz genes which previously were found not modulated 2 days after exploration. Our data show that the expression of Pfn2, Casp3, Pdrg1, Pea15, Ywhaz genes was modulated at 10 or 20 days. The transcript, whose expression had been evaluated with the qRT-PCR, code for proteins which belong to the following functional categories: synaptic modulation, apoptosis, signal transduction. It is interesting to note that the modulation of the expression of these genes was evident some days after environmental exploration, and not previously at 2 days after conditioning as occurred after contextual fear conditioning (CFC). Hence it is possible to hypothesize that the spatial memory processes require a longer period of elaboration than the emotional ones, fundamental for the survival of the species.

中文翻译：

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对新环境的探索导致大鼠中基因表达的调控延长了时间。

在本研究中，我们进行了转录分析，以评估长时间探索引起的基因表达变化。分析是在勘探后3天，10天和20天进行的。我们分析了Pfn2，Casp3，Pdrg1，Pea15，Ywhaz基因表达水平的调控，这些基因先前在探索后两天没有被调控。我们的数据显示，Pfn2，Casp3，Pdrg1，Pea15，Ywhaz基因的表达在10或20天受到调节。转录本的表达已通过qRT-PCR进行了评估，其编码的蛋白质属于以下功能类别：突触调节，细胞凋亡，信号转导。有趣的是，在环境探索后的几天，这些基因表达的调节很明显，而不是之前在进行情境恐惧调理（CFC）之后的2天。因此，有可能假设空间记忆过程比情感过程需要更长的时间，这是物种生存的基础。