Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are present (250 viral RNA copies). Further, the protocol is able to detect viral RNA in saliva samples. The procedure reported could be easily implemented in the generation of a new and sensitive rapid point-of care device for SARS-CoV-2 RNA visual detection.

中文翻译：

---

通过比色环介导的等温扩增快速视觉检测 SARS-CoV-2。


评估了从ORF1ab序列定义的一组新引物的性能，及其与之前发布的N序列引物的组合，通过环介导的等温扩增技术检测 SARS-CoV-2 RNA。 ORF1ab引物组可在 16 分钟内目视检测 SARS-CoV-2 RNA。此外， ORF1ab和N引物同时反应可实现更高的检测灵敏度，特别是在存在少量拷贝（250 个病毒 RNA 拷贝）时。此外，该协议能够检测唾液样本中的病毒 RNA。所报道的程序可以很容易地在生成用于 SARS-CoV-2 RNA 视觉检测的新型、灵敏的快速护理点设备中实施。