SummaryThis study aimed to compare the expression of genes regulating follicles development, survival and steroid hormones secretion in oocytes and granulosa cells (GCs) and study the correlation between their expression and follicular fluid (FF) levels of progesterone (P4) in pregnant and non-pregnant camels. In total, 138 ovarian pairs from slaughtered camels were used. Gene expression and hormonal assay were determined using real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. The obtained results revealed that the number of follicles (3–8 mm) was significantly (P < 0.05) lower in pregnant, compared with non-pregnant, camels. P4 level in the FF was significantly (P < 0.05) higher in pregnant, compared with non-pregnant, camels. However, no significant (P > 0.05) difference was noticed in the oestradiol (E2) level. STAR, PTEN, IGF1 and BCL2 mRNA levels were significantly higher in GCs and significantly lower in oocytes of pregnant, compared with non-pregnant, camels. However, follicle-stimulating hormone receptor (FSHR) mRNA level was significantly lower in GCs and oocytes, and the BMP15 mRNA level was significantly lower in oocytes of pregnant, compared with non-pregnant, camels. P4 level in FF was positively correlated with STAR, PTEN, IGF1 and BCL2 mRNA levels in GCs and negatively correlated with BMP15 mRNA levels in oocytes and FSHR mRNA levels in GCs and oocytes of pregnant camels. It could be concluded that pregnancy-induced variations in oocytes and GC expression of BMP15, IGF1, FSHR, STAR, BCL2, and PTEN genes might be associated with a decrease in the number of follicles and an increase in the FF level of P4.

中文翻译：

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妊娠和未妊娠骆驼卵母细胞、颗粒细胞和卵泡液的分子和激素差异变化

摘要本研究旨在比较卵母细胞和颗粒细胞（GCs）中调节卵泡发育、存活和类固醇激素分泌的基因的表达，并研究它们的表达与妊娠期和非妊娠期卵泡液（FF）孕酮（P4）水平的相关性。怀孕的骆驼。总共使用了来自屠宰骆驼的 138 对卵巢。分别使用实时聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）测定基因表达和激素测定。获得的结果表明，卵泡的数量（3-8 mm）显着（磷< 0.05) 与未怀孕的骆驼相比，怀孕的骆驼较低。FF中的P4水平显着（磷< 0.05) 与未怀孕的骆驼相比，怀孕的骆驼更高。然而，没有显着（磷> 0.05) 在雌二醇 (E2) 水平上存在差异。星星,PTEN,胰岛素样生长因子1和BCL2与未怀孕的骆驼相比，怀孕的 GCs 中的 mRNA 水平显着升高，而在卵母细胞中的 mRNA 水平显着降低。然而，促卵泡激素受体（FSHR) mRNA 水平在 GC 和卵母细胞中显着降低，并且BMP15与未怀孕的骆驼相比，怀孕的卵母细胞中mRNA水平显着降低。FF中的P4水平与星星,PTEN,胰岛素样生长因子1和BCL2GCs中的mRNA水平与BMP15卵母细胞中的 mRNA 水平和FSHR怀孕骆驼的 GC 和卵母细胞中的 mRNA 水平。可以得出结论，妊娠诱导的卵母细胞和 GC 表达的变化BMP15,胰岛素样生长因子1,FSHR,星星,BCL2， 和PTEN基因可能与卵泡数量减少和 P4 FF 水平增加有关。