Although many multifunctional antibacterial materials have been developed, there are few antibacterial materials that can simultaneously monitor pathogenic bacteria and sterilize efficiently. In this work, pH-responsive spiropyran-based fluorinated copolymers with antibacterial, antifouling and fluorescence performances were prepared. First, the random copolymer of methacrylic acid and 1H,1H,2H,2H-perfluorododecyl acrylate (P(MAA-co-FDA)) were synthesized by RAFT polymerization. Second, spiropyran (SP) was grafted onto P(MAA-co-FDA) to obtain the P(MAA-co-SP-co-FDA) copolymer with fluorescence property. At the third step, the remaining carboxyl groups in the grafted copolymer were complexed with protonated polyhexamethylene biguanidine salt (PHMB) to obtain the P(MAA-co-SP-co-FDA)/PHMB copolymer complex antibacterial materials. The results show that the copolymer complex coatings can effectively inhibit the adhesion of bacteria and release PHMB in a bacterial microenvironment to kill S. aureus and E. coli but remain noncytotoxic. The P(MAA-co-SP-co-FDA) copolymer or its complex with PHMB can open the ring and generate a red fluorescence under acidic conditions produced by bacterial growth and metabolism. In addition, the coatings have good biocompatibility, which may be suitable for medical devices to reduce bacterial infection in vivo and in vitro. The prepared multifunctional materials with excellent anti-adhesion, antimicrobial and fluorescence characters will have a broad application prospect in antibacterial, antifouling and monitoring of medical devices.

尽管已经开发了许多多功能抗菌材料，但是很少有能够同时监测病原细菌并有效消毒的抗菌材料。在这项工作中，制备了具有抗菌，防污和荧光性能的pH响应型螺吡喃基氟化共聚物。首先，通过RAFT聚合合成甲基丙烯酸和1H，1H，2H，2H-全氟十二烷基丙烯酸酯的无规共聚物（P（MAA- co -FDA））。其次，将螺吡喃（SP）嫁接到P（MAA- co- SP- co）上以获得P（MAA- co- SP- co-FDA）具有荧光特性的共聚物。在第三步骤中，在该接枝共聚物中的残留羧基用质子化聚双胍盐（PHMB），以获得P（MAA-复合共-Sp-的共-FDA）/ PHMB共聚物复杂抗菌材料。结果表明，该共聚物复合物涂层可以有效抑制细菌的粘附并在细菌微环境中释放PHMB，以杀死金黄色葡萄球菌和大肠杆菌，但仍保持无细胞毒性。P（MAA- co -SP- co-FDA）共聚物或其与PHMB的配合物可在细菌生长和代谢产生的酸性条件下开环并产生红色荧光。另外，涂层具有良好的生物相容性，这可能适合用于医疗器械以减少体内和体外细菌感染。所制备的具有优异的抗粘连，抗菌和荧光特性的多功能材料将在医疗器械的抗菌，防污和监测方面具有广阔的应用前景。