Background

Very early exercise has been reported to exacerbate motor dysfunction; however, its mechanism is largely unknown.

Objective

This study examined the effect of very early exercise on motor recovery and associated brain damage following intracerebral hemorrhage (ICH) in rats.

Methods

Collagenase solution was injected into the left striatum to induce ICH. Rats were randomly assigned to receive placebo surgery without exercise (SHAM) or ICH without (ICH) or with very early exercise within 24 hours of surgery (ICH+VET). We observed sensorimotor behaviors before surgery, and after surgery preexercise and postexercise. Postexercise brain tissue was collected 27 hours after surgery to investigate the hematoma area, brain edema, and Il1b, Tgfb1, and Igf1 mRNA levels in the striatum and sensorimotor cortex using real-time reverse transcription polymerase chain reaction. NeuN, PSD95, and GFAP protein expression was analyzed by Western blotting.

Results

We observed significantly increased skillful sensorimotor impairment in the horizontal ladder test and significantly higher Il1b mRNA levels in the striatum of the ICH+VET group compared with the ICH group. NeuN protein expression was significantly reduced in both brain regions of the ICH+VET group compared with the SHAM group.

Conclusion

Our results suggest that very early exercise may be associated with an exacerbation of motor dysfunction because of increased neuronal death and region-specific changes in inflammatory factors. These results indicate that implementing exercise within 24 hours after ICH should be performed with caution.

中文翻译：

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脑出血后的早期运动康复会促进大脑炎症

背景

据报道，过早运动会加剧运动功能障碍；然而，其机制在很大程度上是未知的。

客观的

本研究检查了极早期运动对大鼠脑出血 (ICH) 后运动恢复和相关脑损伤的影响。

方法

将胶原酶溶液注入左侧纹状体以诱导 ICH。大鼠被随机分配接受无运动的安慰剂手术（SHAM）或无运动的 ICH（ICH）或手术后 24 小时内非常早运动（ICH+VET）。我们观察了术前、术后运动前和运动后的感觉运动行为。术后 27 小时收集运动后脑组织，使用实时逆转录聚合酶链反应研究纹状体和感觉运动皮层中的血肿区域、脑水肿以及Il1b、Tgfb1和Igf1 mRNA 水平。通过蛋白质印迹分析 NeuN、PSD95 和 GFAP 蛋白表达。

结果

与 ICH 组相比，我们观察到水平阶梯测试中熟练感觉运动障碍显着增加，ICH+VET 组纹状体中Il1b mRNA 水平显着升高。与 SHAM 组相比，ICH+VET 组的两个脑区 NeuN 蛋白表达均显着降低。

结论

我们的研究结果表明，由于神经元死亡增加和炎症因子的区域特异性变化，非常早期的运动可能与运动功能障碍的恶化有关。这些结果表明，在 ICH 后 24 小时内进行锻炼应谨慎。