Background

The molecular reclassification of the order Trichosporonales placed the medically relevant Trichosporon species into three genera of the family Trichosporonaceae: Cutaneotrichosporon, Trichosporon, and Apiotrichum. From the clinical and epidemiological standpoint, it is important to identify any species of the family Trichosporonaceae because they present different antifungal susceptibility profiles. In Mexico, little is known about trichosporonosis etiology because the fungi are identified through phenotypic methods.

Aims

To identify at a molecular level 12 yeast isolates morfologically compatible with Trichosporon, obtained from patients with superficial infections.

Methods

The yeast isolates were obtained from patients with white piedra, onychomycosis, and hand and foot dermatomycosis, and were identified morphologically and genotypically (sequencing of the IGS1 region and phylogenetic analysis using the Maximum Likelihood Method). The phylogenetic analysis included 40 yeast sequences from the order Trichosporonales and one from Cryptococcus neoformans as outgroup.

Results

Based on the molecular analysis, we identified three (25%) Trichosporon inkin isolates, two (16.7%) Trichosporon asteroides, two (16.7%) Cutaneotrichosporon mucoides, and one each (8.3%) of Trichosporon aquatile, Trichosporon asahii, Apiotrichum montevideense, Cutaneotrichosporon cutaneum, and Cutaneotrichosporon jirovecii.

Conclusions

The molecular characterization of the isolates showed a broad diversity of species within the order Trichosporonales, particularly among onychomycosis. It is essential to identify these yeasts at the species level to delve into their epidemiology.

中文翻译：

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从引起浅表感染的毛孢子菌目酵母的分子鉴定

背景

Trichosporonales 目的分子重新分类将医学上相关的Trichosporon物种归入Trichosporonaceae科的三个属：Cutaneotrichosporon、Trichosporon和Apiotrichum。从临床和流行病学的角度来看，识别毛孢菌科的任何物种都很重要，因为它们呈现出不同的抗真菌敏感性特征。在墨西哥，对毛孢子​​菌病病因知之甚少，因为真菌是通过表型方法鉴定的。

宗旨

为了在分子水平上鉴定 12 株酵母分离株，在形态学上与从表面感染患者获得的Trichosporon相容。

方法

从患有白斑病、甲真菌病和手足皮肤真菌病的患者中获得酵母分离株，并在形态学和基因型上进行鉴定（IGS1 区域的测序和使用最大似然法进行的系统发育分析）。系统发育分析包括 40 个来自 Trichosporonales 的酵母序列和一个来自作为外群的新型隐球菌。

结果

基于分子分析，我们确定了三个（25％）丝孢inkin分离物，二（16.7％）丝孢星状，二（16.7％）Cutaneotrichosporon mucoides，和中的每一个（8.3％）丝孢aquatile，丝孢asahii，Apiotrichum montevideense，Cutaneotrichosporon cutaneum和Cutaneotrichosporon jirovecii。

结论

分离物的分子特征表明，在毛孢菌目中，特别是在甲真菌病中，物种具有广泛的多样性。在物种水平上识别这些酵母以深入研究它们的流行病学至关重要。