Schwann cells (SCs) play a crucial role in the repair of peripheral nerves. This is due to their ability to proliferate, migrate, and provide trophic support to axon regrowth. During peripheral nerve injury, SCs de-differentiate and reprogram to gain the ability to repair nerves. Cysteine-rich 61 (Cyr61/CCN1) is a member of the CCN family of matrix cell proteins and have been reported to be abundant in the secretome of repair mediating SCs. In this study we investigate the function of Cyr61 in SCs. We observed Cyr61 was expressed both in vivo and in vitro. The promoting effect of Cyr61 on SC proliferation and migration was through autocrine and paracrine mechanisms. SCs expressed αvβ3 integrin and the effect of Cyr61 on SC proliferation and migration could be blocked via αvβ3 integrin. Cyr61 could influence c-Jun protein expression in cultured SCs. In this study, we found that Cyr61 promotes SC proliferation and migration via αvβ3 integrin and regulates c-Jun expression. Our study contributes to the understanding of cellular and molecular mechanisms underlying SC’s function during nerve injury, and thus, may facilitate the regeneration of peripheral nerves after injury.

中文翻译：

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Cyr61通过αvβ3整合素促进雪旺氏细胞增殖和迁移

雪旺氏细胞 (SCs) 在周围神经的修复中起着至关重要的作用。这是由于它们能够增殖、迁移并为轴突再生提供营养支持。在周围神经损伤期间，SC 去分化和重新编程以获得修复神经的能力。富含半胱氨酸的 61 (Cyr61/CCN1) 是基质细胞蛋白 CCN 家族的成员，据报道在修复介导 SCs 的分泌组中含量丰富。在这项研究中，我们调查了 Cyr61 在 SCs 中的功能。我们观察到 Cyr61 在体内和体外均表达。Cyr61 通过自分泌和旁分泌机制促进 SC 增殖和迁移。SCs 表达 αvβ3 整合素，Cyr61 对 SC 增殖和迁移的影响可以通过 αvβ3 整合素阻断。Cyr61 可以影响培养的 SCs 中 c-Jun 蛋白的表达。在本研究中，我们发现 Cyr61 通过 αvβ3 整合素促进 SC 增殖和迁移并调节 c-Jun 表达。我们的研究有助于了解神经损伤期间 SC 功能背后的细胞和分子机制，从而可能促进损伤后周围神经的再生。