Abstract

Lasianthera africana P. Beauv. (Icacinaceae) is a good source of natural antioxidants, having the potential to protect against oxidative stress-related diseases and complications. This study investigated the antioxidant, hepatoprotective and curative effects of flavonoid-rich fraction of L. africana leaves (LAFRF) against carbon tetrachloride-induced hepatotoxicity in Wistar rats. Phytochemical, nutrient content, and in vitro antioxidant activity of LAFRF were determined by standard methods. Fifty Wistar rats were randomized into 10 groups (n = 5). Groups 1 and 2 served as normal and CCl₄ controls, respectively. Groups 3A–6A constituted the protective study while groups 3B–6B represented the curative study. The effects of LAFRF at 3, 10, and 30 mg/kg body weight (b.w.) on lipid peroxidation, antioxidant status, liver enzymes activities, and histology of CCl₄-intoxicated rats were assessed. LAFRF total flavonoids (281.05 ± 7.44 mg QE/g), indicated LD₅₀ above 5000 mg/kg b.w., and scavenged ABTS*⁺ with an IC₅₀ of 5.05 ± 0.00 µg/mL relative to butylated hydroxytoluene (4.16 ± 0.00 µg/mL), and a concentration-dependent increase in total antioxidant capacity. Carbon tetrachloride (1 mL/kg) triggered significant (p < 0.05) increases in malonedialdehyde concentration (2.67 ± 0.21 mg/mL), with a corresponding decline in antioxidant status, and increases in alkaline phosphatase, alanine and aspartate aminotransferase activities (68.00 ± 9.59 IU/L, 79.60 ± 5.03 IU/L and 81.80 ± 3.96 IU/L), respectively. LAFRF significantly (p < 0.05) lowered lipid peroxidation levels, liver enzyme activities, increased antioxidant status, and improved hepatic histo-architecture of pre- and post LAFRF-treated rats. This demonstrates its high antioxidative, hepatoprotective and curative effects, indicating its potential for future drug development.

摘要

Lasianthera Africana P. Beauv。(Icacinaceae) 是天然抗氧化剂的良好来源，具有预防氧化应激相关疾病和并发症的潜力。本研究调查了非洲乳香叶中富含类黄酮的部分 (LAFRF) 对四氯化碳诱导的 Wistar 大鼠肝毒性的抗氧化、保肝和治疗作用。通过标准方法测定 LAFRF 的植物化学成分、营养成分和体外抗氧化活性。将 50 只 Wistar 大鼠随机分为 10 组（n  = 5）。第 1 组和第 2 组作为正常组和 CCl ₄分别控制。3A-6A 组构成保护性研究，而 3B-6B 组代表治疗性研究。评估了 3、10 和 30 mg/kg 体重 (bw) 的LAFRF对 CCl _{4中毒大鼠的脂质过氧化、抗氧化状态、肝酶活性和组织学的影响。}LAFRF 总黄酮 (281.05 ± 7.44 mg QE/g)，表明 LD ₅₀高于 5000 mg/kg bw，并清除 ABTS* ⁺ ，相对于丁基羟基甲苯 (4.16 ± 0.00 µg/mL)的 IC ₅₀为 5.05 ± 0.00 µg/mL )，以及总抗氧化能力的浓度依赖性增加。四氯化碳 (1 mL/kg) 引发显着 ( p < 0.05) 丙二醛浓度增加 (2.67 ± 0.21 mg/mL)，抗氧化状态相应下降，碱性磷酸酶、丙氨酸和天冬氨酸氨基转移酶活性增加 (68.00 ± 9.59 IU/L、79.60 ± 5.03 IU/L 和81.80 ± 3.96 IU/L)，分别。LAFRF 显着 ( p  < 0.05) 降低了 LAFRF 治疗前后大鼠的脂质过氧化水平、肝酶活性、抗氧化状态增加，并改善了肝脏组织结构。这证明了其高抗氧化、保肝和治疗作用，表明其在未来药物开发中的潜力。