To increase the efficiency of interpretation of mast cell’s contribution to the state of a specific tissue microenvironment, it is necessary to detail the molecular composition of their secretome and analyze the pathways of degranulation. Developed method of combining immunomorphological and histochemical staining protocols contributes to the most objective detection of the integral level of tryptase expression in the intraorgan population of the skin mast cells. Novel technique for tryptase detection expands the possibilities of morphological analysis, provides researchers with additional data on the structure of the mast cell population and helps visualize the processing and cytological features and structural targets of tryptase during the development of adaptive and pathological reactions. Objective determination of the tryptase profile for organ-specific mast cell populations is in great demand in clinical practice for the interpretation of pathological processes, including inflammation and oncogenesis.

为了提高对肥大细胞对特定组织微环境状态贡献的解释效率，有必要详细说明其分泌组的分子组成并分析脱粒途径。结合免疫形态学和组织化学染色方案的开发方法有助于最客观地检测皮肤肥大细胞器官内群体中类胰蛋白酶表达的整体水平。用于类胰蛋白酶检测的新技术扩展了形态学分析的可能性，为研究人员提供了关于肥大细胞群结构的额外数据，并有助于在适应性和病理反应的发展过程中可视化类胰蛋白酶的加工和细胞学特征以及结构目标。