Invertebrates change the metabolite concentrations in their bodies to adapt to environmental salinity. The kuruma shrimp Marsupenaeus japonicus is one such invertebrate. It lives in coastal areas and is also known to change free amino acid concentrations depending on environmental salinity. To examine the relationship between the concentrations of metabolites, including amino acids, in the kuruma shrimp with environmental salinity, metabolome analysis was performed on the abdominal muscle of shrimps acclimated at 17, 34 and 40‰ salinity for 24 h. Principal component analysis revealed that the accumulation patterns of metabolites using the 111 metabolites detected in the shrimps exposed to different salinities were depicted in a salinity-dependent manner. The concentrations of alanine and glutamine were increased following exposure to increasing levels of salinity, suggesting that these free amino acids function in intracellular osmoregulation of the kuruma shrimp. Furthermore, the concentration of glycolytic metabolites was significantly decreased at high salinity. The concentrations of most of the metabolites related to the tricarboxylic acid (TCA) cycle also tended to decrease at high salinity. Considering the levels of expression of the genes related to various metabolic pathways, it seems that glycolysis is accelerated at high salinity.

无脊椎动物改变其体内代谢产物的浓度以适应环境盐度。库鲁玛虾Marsupenaeus japonicus就是这样一种无脊椎动物。它生活在沿海地区，并且还已知会根据环境盐度改变游离氨基酸的浓度。为了研究环境盐度对黑熊虾的代谢物浓度（包括氨基酸）之间的关系，对盐度为17、34和40‰的虾的腹部肌肉进行了24小时的代谢组分析。主成分分析表明，利用盐分依赖的方式描述了在暴露于不同盐分的虾中检测到的111种代谢物的代谢物积累模式。暴露于盐度水平升高后，丙氨酸和谷氨酰胺的浓度增加，表明这些游离氨基酸在库鲁玛虾的细胞内渗透调节中起作用。此外，在高盐度条件下，糖酵解代谢产物的浓度显着降低。在高盐度下，与三羧酸（TCA）循环有关的大多数代谢物的浓度也趋于降低。考虑到与各种代谢途径有关的基因的表达水平，似乎在高盐度下糖酵解被加速了。