Pharmaceutical drugs are among the most used chemicals for human and veterinary medicines, aquaculture and agriculture. Pharmaceuticals are environmentally persistent, biologically active molecules, thereby having the potential to exert biological effects on non-target species. Among the most used pharmaceuticals, one may find salicylic acid (SA), a non-steroid anti-inflammatory drug (NSAID) that acts by inhibiting the enzymes cyclooxigenases; it is also possible to identify acetazolamide (ACZ), a diuretic that acts by inhibiting the activity of carbonic anhydrase (CA). In this work, the effects of both single and combined effects of these drugs were assessed on the marine gastropod Phorcus lineatus, by measuring key enzymatic activities, namely carbonic anhydrase (CA) and cyclooxygenase (COX), under two different exposure periods (14 and 28 days). We observed no straightforward pattern of enzymatic response in all treatments of both pharmaceuticals, on both analyzed tissues (gut and gills), and for both exposure regimes. We assume that this species is not responsive to the hereby tested pharmaceuticals, a finding that may be due to general mechanisms of response to adverse conditions, such as reduction of metabolism, of heart rate, of filtration rates, and to the increase production of mucus. All these functional adaptations can mitigate the deleterious effects caused by adverse conditions, without triggering biochemical responses. In conclusion, the species P. lineatus seems not to be sensitive in terms of these specific enzymatic pathways to these contaminants, under the adopted conditions.

药物是人类和兽药，水产养殖和农业中最常用的化学药品之一。药物是环境持久的生物活性分子，因此具有对非目标物种产生生物学效应的潜力。在最常用的药物中，人们可能发现了水杨酸（SA），这是一种非甾体类抗炎药（NSAID），可通过抑制环氧化酶的作用发挥作用。还可以鉴定出乙酰唑胺（ACZ），一种通过抑制碳酸酐酶（CA）的活性而起作用的利尿剂。在这项工作中，评估了这些药物在海洋腹足纲Phorcus lineatus上的单一或联合作用通过在两个不同的暴露时间段（14天和28天）下测量关键的酶活性，即碳酸酐酶（CA）和环氧合酶（COX）。我们在两种药物的所有处理中，在被分析的组织（肠和g）上以及在两种暴露方式下均未观察到酶促反应的简单模式。我们认为该物种对特此测试的药物没有反应，这一发现可能是由于对不良条件的一般反应机制引起的，例如对新陈代谢的降低，心率，滤过率的降低以及粘液产生的增加。所有这些功能调整都可以减轻不利条件引起的有害影响，而不会触发生化反应。总之，P。lineatus物种 在采用的条件下，对于这些污染物的这些特定酶促途径似乎并不敏感。