Wild pansy (Viola tricolor L.) is of the Violaceae plant family. Some of the medicinal properties of this plant are related to the ability to produce and biosynthesized a group of flavonoids. Silver nanoparticles are one of the most important and attractive nanomaterials among several metal nanoparticles.In the present study, plant-mediated nanosilver was produced from aqueous extract of Pansy flower as a good covering and stabilizing agent using the green synthesis method. Dynamic light scattering (DLS), transmission electron microscopy (TEM), and zeta potential methods were used to determine the specifications of synthesized nanoparticles. In this study, the effect of different concentrations of bio nanosilver 0, 10, 50, and 100 mg/l on phytochemical and physiological properties, and the expression of some key genes involved in flavonoids biosynthesis including phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavonoid 3′, 5′-hydroxylase (F3′5′H), and flavonoid 3′-hydroxylase (F3′H) were determined in V. tricolor plant. The results showed nanosilver treatments cause the accumulation of manganese, zinc, and silver elements and increasing the content of phenolic, flavonoid, anthocyanin and antioxidant activity. Using AgNPs also causes the increase in activity of antioxidant enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), phenylalanine ammonia-lyase (PAL), and peroxidase (POD). Besides, treatment with a concentration of 10 mg/l of silver nanoparticles significantly causes an increase in flavonoids compounds such as rutin, apigenin, and quercetin. Our results suggest that bio nanosilver could increase secondary metabolites content in the V. tricolor plant.

三色堇（三色堇）L.）是堇菜科植物家族的一员。这种植物的某些药用特性与产生和生物合成一组类黄酮的能力有关。银纳米颗粒是几种金属纳米颗粒中最重要和最具吸引力的纳米材料之一。动态光散射（DLS），透射电子显微镜（TEM）和zeta电位方法用于确定合成纳米粒子的规格。在这项研究中，不同浓度的生物纳米银0、10、50和100 mg / l对植物化学和生理特性的影响，PAL），查耳酮合酶（CHS），类黄酮3'，5'-羟化酶（F3'5'H），和类黄酮3 '羟化酶（F3'H）的测定V.三色植物。结果表明，纳米银处理引起锰，锌和银元素的积累，并增加了酚，类黄酮，花色苷的含量和抗氧化活性。使用AgNPs还会导致抗氧化剂酶（例如超氧化物歧化酶（SOD），抗坏血酸过氧化物酶（APX），苯丙氨酸氨裂合酶（PAL）和过氧化物酶（POD））的活性增加。此外，浓度为10 mg / l的银纳米颗粒的处理显着增加了类黄酮化合物（如芦丁，芹菜素和槲皮素）的含量。我们的结果表明，生物纳米银可以增加三色堇植物中次生代谢产物的含量。