In March 2017, a wild‐caught female common mudpuppy Necturus maculosus from Iowa, USA, with an enlarged posterior abdomen was submitted for diagnostic assessment. The cause of the abdominal distension was a large fluid‐filled abdominal mass, diagnosed as a nephroblastoma. Parasites and numerous bacteria were isolated and identified from the mudpuppy but were determined to be incidental. Samples of the neoplasm inoculated onto an American toad Anaxyrus americanus cell line (BufoTad) yielded cytopathic effect during several passages. However, standard molecular testing of the cell culture supernatant failed to identify any viruses. Next‐generation sequencing identified the replicating agent as a bacterium of the genus Acholeplasma. Immunohistochemistry confirmed the presence of Acholeplasma within the nephroblastoma, including within tumor cells. This is the first report of nephroblastoma and the second report of neoplasia in this species. The results also suggest that certain bacteria of the genus Acholeplasma might be oncogenic.

中文翻译：

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一种常见的 Mudpuppy Necturus maculosus 中的肾母细胞瘤与无胆原体属的软疣细菌同时存在

2017 年 3 月，一只来自美国爱荷华州的野生捕捞的雌性普通泥巴狗Necturus maculosus被提交进行诊断评估。腹胀的原因是腹部巨大的充满液体的肿块，诊断为肾母细胞瘤。从泥巴狗身上分离并鉴定出寄生虫和许多细菌，但被确定是偶然的。接种到美洲蟾蜍Anaxyrus americanus细胞系 (BufoTad)上的肿瘤样本在几次传代过程中产生了细胞病变效应。然而，细胞培养上清液的标准分子测试未能识别出任何病毒。下一代测序将复制剂鉴定为无胆原体属细菌. 免疫组织化学证实了肾母细胞瘤内（包括肿瘤细胞内）存在无胆原体。这是肾母细胞瘤的第一份报告，也是该物种肿瘤形成的第二份报告。结果还表明，无胆原体属的某些细菌可能具有致癌性。