Matrix metalloproteinase (MMP)-9 belongs to the zinc-dependent endopeptidase family, which is associated with important inflammatory processes and plays an important role in cell metabolism, embryonic development, and wound repair. The present study was performed using Pampus argenteus individuals fed with different feeds. The full-length cDNA of the MMP-9 gene was 2783 bp and encoded 681 amino acids. The amino acid sequence analysis of Pampus argenteus MMP-9 showed that it contained a highly conserved fibronectin type II domain and heme domain, and it was evolutionarily closely related to the MMP-9 of Seriola dumerili. Real-time PCR, western blotting, and enzyme activity assay were used to analyze the expression of MMP-9 in P. argenteus under different feeding conditions, in various tissues, and on different feeding days. The results showed that the expression of MMP-9 in group A (fed with jellyfish for the first 20 days) and B’ group (fed with jellyfish for subsequent 15 days) was significantly higher than that in other groups (P < 0.05) and the expression in the kidney was significantly higher than that in other tissues (P < 0.05). The expression increased gradually with the increase in feeding time. The results suggest that MMP-9 expression is induced in P. argenteus fed with jellyfish and plays a very important role in the immune response of the body.

基质金属蛋白酶（MMP）-9属于锌依赖性内肽酶家族，与重要的炎症过程相关，在细胞代谢，胚胎发育和伤口修复中起重要作用。本研究是使用饲喂不同饲料的南美白individuals（Pampus argenteus）个体进行的。MMP-9基因的全长cDNA为2783 bp，编码681个氨基酸。南美白PMMP -9的氨基酸序列分析表明，它含有高度保守的II型纤连蛋白结构域和血红素结构域，并且在进化上与Seriola dumerili的MMP-9密切相关。实时荧光定量PCR，蛋白质印迹和酶活性测定用于分析MMP-9在肝细胞中的表达。在不同的饲养条件下，在不同的组织中以及在不同的饲养天数下，阿根廷假单胞菌。结果表明，A组（前20天喂海el）和B'组（后15天喂海el）中MMP-9的表达明显高于其他各组（P <0.05），且肾脏中的表达明显高于其他组织中的表达（P <0.05）。表达随着进食时间的增加而逐渐增加。结果表明，MMP-9表达在水母喂养的阿根廷对虾中被诱导，并且在机体的免疫反应中起着非常重要的作用。