Aims To quantify retinal vascular bed area (RVBA) in square millimetres on stereographically projected ultra-wide field (UWF) fluorescein angiography (FA) in eyes with diabetic retinopathy (DR). Methods A prospective, observational study. Baseline Optos 200Tx UWF FA images of 80 eyes with DR from the DAVE ([NCT01552408][1]) and RECOVERY ([NCT02863354][2]) studies were stereographically projected at the Doheny Image Reading Center to adjust for peripheral distortion. The early-phase FA frame was used to extract the retinal vasculature as a mask for calculating RVBA. The pixels of the retinal vasculature were automatically computed in square millimetres using manufacturer-provided software. Results Eighteen of 80 diabetic eyes were excluded because image quality and contrast were insufficient for automatic extraction of the retinal vasculature from the background fluorescence. The remaining 62 eyes were included in the final analysis. In comparison with age-matched and sex-matched normal controls, eyes with DR had a higher global RVBA for the entire retina (p<0.001), and RVBA correlated with DR severity (p<0.001), with a higher RVBA in eyes with proliferative DR (66.1±16.2 mm2) than in those with non-proliferative DR (56.2±16.6 mm2) or in normal controls (37.2±9.9 mm2). This tendency was also present in the posterior retina and mid-periphery but absent in the far-periphery. RVBA did not correlate with retinal ischaemia (p>0.05). Conclusions Eyes with DR harboured a larger global RVBA for the entire retina than normal controls, and RVBA appeared to indicate DR severity. However, this biomarker was not observed to be a good indicator of retinal ischaemia. All data relevant to the study are included in the article or uploaded as online supplemental information. The data are available from WF (wfanacademic@gmail.com). [1]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT01552408&atom=%2Fbjophthalmol%2F106%2F8%2F1126.atom [2]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT02863354&atom=%2Fbjophthalmol%2F106%2F8%2F1126.atom

中文翻译：

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超广角荧光血管造影的视网膜血管床面积指示糖尿病视网膜病变的严重程度

目的 量化糖尿病视网膜病变 (DR) 眼的立体投影超广角 (UWF) 荧光素血管造影 (FA) 中的视网膜血管床面积 (RVBA)（以平方毫米为单位）。方法 一项前瞻性、观察性研究。来自 DAVE ([NCT01552408][1]) 和 RECOVERY ([NCT02863354][2]) 研究的 80 只眼睛的基线 Optos 200Tx UWF FA 图像被立体投影到 Doheny 图像读取中心，以调整周边失真。早期 FA 框架用于提取视网膜脉管系统作为计算 RVBA 的掩码。使用制造商提供的软件以平方毫米为单位自动计算视网膜脉管系统的像素。结果 80 只糖尿病眼中有 18 只被排除在外，因为图像质量和对比度不足以从背景荧光中自动提取视网膜脉管系统。其余 62 只眼纳入最终分析。与年龄匹配和性别匹配的正常对照相比，患有 DR 的眼睛的整个视网膜的整体 RVBA 更高（p<0.001），并且 RVBA 与 DR 严重程度相关（p<0.001），患有 DR 的眼睛的 RVBA 更高增殖性 DR (66.1±16.2 mm2) 高于非增殖性 DR (56.2±16.6 mm2) 或正常对照组 (37.2±9.9 mm2)。这种趋势也存在于视网膜后部和中部周边，但在远周边不存在。RVBA 与视网膜缺血无关（p>0.05）。结论 DR 患者的整个视网膜比正常对照组的整体 RVBA 更大，并且 RVBA 似乎表明 DR 严重程度。然而，没有观察到这种生物标志物是视网膜缺血的良好指标。所有与研究相关的数据都包含在文章中或作为在线补充信息上传。数据可从 WF (wfanacademic@gmail.com) 获得。[1]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT01552408&atom=%2Fbjophthalmol%2F106%2F8%2F1126.atom [2]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT02863354&atom=%2Fbjophthalmol%2F106%2F8 %2F1126.atom 所有与研究相关的数据都包含在文章中或作为在线补充信息上传。数据可从 WF (wfanacademic@gmail.com) 获得。[1]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT01552408&atom=%2Fbjophthalmol%2F106%2F8%2F1126.atom [2]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT02863354&atom=%2Fbjophthalmol%2F106%2F8 %2F1126.atom 所有与研究相关的数据都包含在文章中或作为在线补充信息上传。数据可从 WF (wfanacademic@gmail.com) 获得。[1]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT01552408&atom=%2Fbjophthalmol%2F106%2F8%2F1126.atom [2]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT02863354&atom=%2Fbjophthalmol%2F106%2F8 %2F1126.atom