Abstract—

The expression of the genes encoding the inhibitors of serine (ISP) and cysteine ​​proteinases (ICP) was studied in the roots of tomato plants resistant and susceptible to the root-knot nematode Meloidogyne incognita during infection and under the effects of signaling molecules: salicylic (SA) and jasmonic (JA) acids. It was shown that, upon infection, resistant plants are characterized by an increased accumulation of transcripts of the ICP and ISP genes at the stages of penetration and development in the roots, while the level of transcription does not change in susceptible plants. There was a significant decrease in nematode invasion in susceptible plants after treatment with SA or JA compared to untreated plants, which makes it possible to determine the role of the studied proteinase inhibitors in resistance induced by signaling molecules. It was revealed that an increase in expression of the genes of proteinase inhibitors is accompanied by inhibition of the reproductive potential and size of M. incognita females, as well as by a decrease in plant infection.

中文翻译：

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根结线虫根结线虫侵染番茄植株中蛋白酶抑制剂的基因表达及其对水杨酸和茉莉酸活性的调节

摘要-

研究了在感染过程中以及在信号分子的作用下对根结线虫Meloidogyne incognita有抗性和敏感性的番茄植株的根中，编码丝氨酸（ISP）和半胱氨酸蛋白酶（ICP）抑制剂的基因的表达：水杨酸（SA）和茉莉酸（JA）酸。结果表明，感染后，抗性植物的特征是ICP和ISP转录本的积累增加基因在根部的渗透和发育阶段，而转录水平在易感植物中不会改变。与未处理的植物相比，用SA或JA处理后，易感植物中线虫的入侵显着减少，这使得确定所研究的蛋白酶抑制剂在信号分子诱导的抗性中的作用成为可能。揭示了蛋白酶抑制剂基因表达的增加伴随着隐孢子虫雌性的繁殖潜力和大小的抑制，以及植物感染的减少。