Abstract

The physiological changes in G. mellonella larvae treated with unirradiated and 2 Gy gamma irradiated S. carpocapsae had been investigated. The results indicated that S. carpocapsae exhibited a significant decrease in the total body concentrations of proteins, lipids, carbohydrates and urea of 5th larval of G. mellonella with increasing the infection incubation period. Moreover, the decrease in the concentration of these total body metabolic products was greatly remarkable in larvae infected with gamma irradiated S. carpocapsae. In contrast, the results revealed that S. carpocapsae caused a significant increase in the acid phospatase and chitinase activity of the total body of 5th larval instar of G. mellonella with increasing the treatment time. Which was greatly remarkable in larvae treated with gamma irradiated S. carpocapsae. Protein bands at different time intervals for all tested treatments revealed the absence of some bands, appearance of new bands and changes in the intensity of the bands. Therefore, it could be concluded that gamma irradiation of S. carpocapsae increased it’s efficacy and can be used in pest control.

中文翻译：

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伽玛辐照的Steinernema carpocapsae对虫幼虫某些生理方面的影响

摘要

研究了未经辐照和2 Gyγ辐照的S. carpocapsae处理的G. mellonella幼虫的生理变化。结果表明，S. carpocapsae表现出的蛋白质，脂质，碳水化合物和的第五幼虫的尿素的总身体浓度显著降低蜡螟随着感染潜伏期。而且，这些全身代谢产物的浓度的降低在受γ射线辐照的沙门氏菌感染的幼虫中非常显着。相比之下，结果表明，沙门氏菌（S. carpocapsae）随着治疗时间的延长，其引起了G. mellonella幼虫5龄幼虫的全身酸性磷酸酶和几丁质酶活性的显着增加。这在用γ射线辐照的S. carpocapsae处理的幼虫中极为明显。对于所有测试的治疗，在不同时间间隔的蛋白条带显示不存在某些条带，出现了新的条带以及条带强度的变化。因此，可以得出结论，伽玛射线辐照炭荚膜链球菌可提高其功效，可用于害虫防治。