Lygodium microphyllum (Cav.) R. Br. (Schizaeales: Lygodiaceae) is among the most damaging invasive plant species in Florida, USA. Following mechanical and herbicide treatment, the plant typically recovers to pre-treatment levels within 1–2 years through regrowth from the adult rhizome and recruitment of new sporelings. A classical biocontrol agent, the gall-inducing mite Floracarus perrepae Knihinicki & Boczek (Acariformes: Eriophyidae), could attack recovering L. microphyllum, thereby reducing the need for repeated treatments. Here, we investigated natural F. perrepae colonization of recovering L. microphyllum following treatment using two field experiments. First, we monitored F. perrepae colonization on adult regrowth and sporelings on tree islands that were clipped and treated with herbicide (glyphosate or triclopyr). At 24 months post-treatment, mite galls were most abundant on glyphosate treated islands where > 50% of adult rachises and > 80% of sporelings exhibited galls and were rare on untreated islands. Second, we investigated the separate effects of clipping and herbicide (triclopyr) treatment on F. perrepae colonization of regrowth within small-scale plots at four heavily invaded sites. Galls were most prevalent following cutting and least prevalent following herbicide seven months post-treatment, but, on a per pinna (i.e., leaflet) basis, were equivalent across treatments. Taken together, we report that both L. microphyllum regrowth and especially sporelings were highly susceptible to colonization by F. perrepae. Our findings suggest that biological control of L. microphyllum is compatible with herbicide treatment, and future research is needed to determine how best to integrate these management tactics.

小叶忍冬（Rygodium microphyllum）（Cav。）（Schizaeales：Lygodiaceae）是美国佛罗里达州最具破坏性的入侵植物之一。经过机械和除草剂处理后，植物通常会在成年根状茎长出并招募新的幼芽后，在1-2年内恢复到预处理水平。一种经典的生物防治剂，可引起gall虫的螨类弗洛拉克鲁斯菌（Floracarus perrepae） Knihinicki＆Boczek（Acariformes：Eriophyidae）可以攻击恢复的小叶利什曼原虫，从而减少了重复治疗的需要。在这里，我们使用两个田间实验研究了自然百日草定植的恢复小叶柳桉的定殖。首先，我们监测了F. perrepae成虫在成年后生长，并在小岛上被孢子剥落，并用除草剂（草甘膦或草lop膦）处理。处理后24个月，在草甘膦处理过的岛上，螨虫的胆汁最为丰富，其中> 50％的成年羽扇豆和> 80％的孢子芽表现出胆汁，在未经处理的岛上很少见。其次，我们研究了剪草和除草剂（草lop虫）处理对四个重度侵袭的小规模小块土地上培植的F. perrepae的再生长的单独影响。在处理后七个月，cutting虫在切割后最盛行，而在除草剂后最不盛行，但在每个品系（即小叶）的基础上，各处理之间的虫是等效的。两者合计，我们报告两个小叶柳再生菌（尤其是孢子）极易受到F. perrepae的侵染。我们的发现表明，对小叶李斯特菌的生物防治与除草剂处理兼容，还需要进一步的研究来确定如何最好地整合这些管理策略。