Corchorus capsularis, commonly known as jute occupies the leading position in the production of natural fibre alongside lower environmental threat. Small noncoding ~21 to 24 nucleotides long microRNAs play significant roles in regulating the gene expression as well as different functions in cellular growth and development. Here, the study adopted a comprehensive in silico approach to identify and characterize the conserved miRNAs in the genome of C. capsularis including functional annotation of specific gene targets. Expressed Sequence Tags (ESTs) based homology search of 3350 known miRNAs of dicotyledons were allowed against 763 non-redundant ESTs of jute genome, resulted in the prediction of 5 potential miRNA candidates belonging five different miRNA families (miR1536, miR9567-3p, miR4391, miR11300, and miR8689). The putative miRNAs were composed of 18 nucleotides having a range of -0.49 to -1.56 MFEI values and 55%–61% of (A + U) content in their pre-miRNAs. A total of 1052 gene targets of putative miRNAs were identified and their functions were extensively analyzed. Most of the gene targets were involved in plant growth, cell cycle regulation, organelle synthesis, developmental process and environmental responses. Five gene targets, namely, NAC Domain Containing Protein, WRKY DNA binding protein, 3-dehydroquinate synthase, S-adenosyl-L-Met–dependent methyl transferase and Vascular-related NAC-Domain were found to be involved in the lignin biosynthesis, phenylpropanoid pathways and secondary wall formation. The present study might accelerate the more miRNA discovery, strengthening the complete understanding of miRNAs association in the cellular basis of lignin biosynthesis towards the production of high standard jute products.

俗称黄麻的Corcorrus荚ris在天然纤维生产中占据领先地位，同时对环境的威胁也较小。小的非编码〜21至24个核苷酸长的microRNA在调节基因表达中起重要作用，并在细胞生长和发育中发挥不同作用。在这里，这项研究采用了全面的计算机方法，以鉴定和表征荚膜梭菌基因组中的保守miRNA 。包括特定基因靶标的功能注释。允许对3350个双子叶植物已知miRNA进行基于表达序列标签（EST）的同源性搜索，以对黄麻基因组的763个非冗余ESTs进行预测，从而预测了5个可能的miRNA候选物，它们属于五个不同的miRNA家族（miR1536，miR9567-3p，miR4391， miR11300和miR8689）。假定的miRNA由18个核苷酸组成，其前miRNA中的MFEI值在-0.49至-1.56之间，并且（A + U）含量的55％-61％。总共确定了1052个推定的miRNA的基因靶标，并对其功能进行了广泛的分析。大多数基因靶标涉及植物生长，细胞周期调控，细胞器合成，发育过程和环境响应。五个基因靶标，即NAC域含蛋白，WRKY DNA结合蛋白，发现3-脱氢喹啉合酶，S-腺苷-L-Met依赖性甲基转移酶和与血管相关的NAC-Domain参与了木质素的生物合成，苯丙烷途径和次生壁形成。本研究可能会加速更多miRNA的发现，从而增强木质素生物合成的细胞基础上miRNA缔合对高标准黄麻产品生产的完整理解。