The mycotoxin Ochratoxin A (OTA) is responsible for producing many effects on human and animal health. In this work, the evaluation of the presence of OTA in tea beverage samples consisted of extraction and preconcentration through the solidification of a floating organic drop (DLLME-SFO) combined with an additional octadecyl silane clean-up step. The obtained extract was analyzed by UHPLC-MS/MS. Interferences from the matrix were effectively reduced and, consequently, recovery increased from 43.18% ± 4.1%–96.02% ± 2.54%. The validation assays were carried out by external calibration and spiked samples, with satisfactory recoveries. An adequate dynamic calibration range was obtained over a concentration interval between 0.5 and 70 μg mL⁻¹ OTA. Capabilities of detection and quantification were 0.5 and 1.4 μg mL⁻¹. The obtained Green Certificate was compared with other techniques to establish the greenness profile of the procedure. Quantification of ochratoxin A levels in tea samples was performed.

霉菌毒素赭曲霉毒素 A (OTA) 对人类和动物健康产生多种影响。在这项工作中，茶饮料样品中 OTA 存在的评估包括通过漂浮有机液滴 (DLLME-SFO) 的固化进行提取和预浓缩，并结合额外的十八烷基硅烷净化步骤。通过UHPLC-MS/MS分析获得的提取物。基质的干扰得到有效减少，回收率从 43.18% ± 4.1% 提高到 96.02% ± 2.54%。通过外部校准和加标样品进行验证测定，回收率令人满意。在0.5和70 μg mL ^-1 OTA之间的浓度区间内获得了足够的动态校准范围。检测和定量能力为0.5和1.4μgmL ^-1 。将获得的绿色证书与其他技术进行比较，以确定该程序的绿色概况。对茶样品中的赭曲霉毒素 A 水平进行了定量。