Protein structures are directly linked to biological functions. However, there is a gap of knowledge between the decoded genome and the structure. To bridge the gap, we focused on the secondary structure (SS). From a comprehensive analysis of predicted SS of proteins in different types of organisms, we have arrived at the following findings: The proportions of SS in genomes were different among phylogenic domains. The distributions of strand lengths indicated structural limitations in all of the species. Different from bacteria and archaea, eukaryotes have an abundance of α-helical and random coil proteins. Interestingly, there was a relationship between SS and post-translational modifications. By calculating hydrophobicity moments of helices and strands, highly amphipathic fragments of SS were found, which might be related to the biological functions. In conclusion, comprehensive predictions of SS will provide valuable perspectives to understand the entire protein structures in genomes and will help one to discover or design functional proteins.

蛋白质结构与生物学功能直接相关。然而，解码的基因组和结构之间存在知识差距。为了弥合差距，我们专注于二级结构（SS）。通过对不同类型生物体中蛋白质的预测SS的综合分析，我们得出以下发现： SS在基因组中的比例在系统发育域之间存在差异。链长度的分布表明所有物种的结构限制。与细菌和古生菌不同，真核生物具有丰富的α-螺旋和无规卷曲蛋白。有趣的是，SS 和翻译后修饰之间存在关系。通过计算螺旋和链的疏水性矩，发现了高度两亲的 SS 片段，这可能与生物学功能有关。总之，SS的综合预测将为理解基因组中的整个蛋白质结构提供有价值的视角，并将有助于发现或设计功能性蛋白质。