In our previous study, all Pseudomonas strains THP6, THP41, and OHP5 were identified as fluoride-resistant bacteria isolated from Dindigul district, Tamilnadu, India. The selected strains exhibiting a high level of fluoride resistance was determined in Luria broth (LB) medium and LB agar plates. In a further effort, fluoride-resistant organisms were tested for hemolytic activity and showed β-hemolysis on blood agar plates. The virulence factors such as gyrB, toxA, algD and lasB, plcH, rhlC and biofilm response genes (pslA, pelA, ppyR) were detected by PCR analysis. The putative genus-specific and species-specific PCR also confirmed that the selected fluoride-resistant strains were belonging to Pseudomonas aeruginosa species. Fluoride-resistance gene crcB was amplified by gene-specific primers. The crcB gene was cloned in TA vector and transformed into E. coli DH5α. Comparative and blast analysis of THP6, THP41, and OHP5 strains crcB gene sequences were high homology with P. aeruginosa fluoride efflux transporter crcB and P. aeruginosa putative fluoride ion transporter crcB. The recombinants were efficiently growing in the NaF containing LB agar plates. The fluoride tolerance of these strains was also associated with resistance to multiple antibiotics. These results can lead to the use of the fluoride resistance gene of P. aeruginosa for the development of a biosensor for fluoride detection.

在我们之前的研究中，所有假单胞菌菌株 THP6、THP41 和 OHP5 都被鉴定为从印度泰米尔纳德邦 Dindigul 区分离的耐氟细菌。在 Luria 肉汤 (LB) 培养基和 LB 琼脂平板中确定了表现出高水平氟化物抗性的所选菌株。在进一步的努力中，对耐氟生物进行了溶血活性测试，并在血琼脂平板上显示了 β-溶血。gyrB , toxA , algD和lasB , plcH , rhlC和生物膜反应基因( pslA , pelA , ppyR )等毒力因子) 通过 PCR 分析检测到。假定的属特异性和种特异性 PCR 也证实所选的耐氟菌株属于铜绿假单胞菌属。通过基因特异性引物扩增抗氟基因crcB。在重庆农商行基因TA载体克隆并转化为ē。大肠杆菌DH5α。THP6、THP41 和 OHP5 菌株crcB基因序列的比较和爆炸分析与P高度同源。铜绿假单胞菌氟流出转运重庆农商行和P。铜绿假单胞菌氟离子转运蛋白CRC B . 重组体在含有 NaF 的 LB 琼脂平板中有效生长。这些菌株的氟耐受性也与对多种抗生素的抗性有关。这些结果可以导致使用P的氟化物抗性基因。aeruginosa用于开发用于氟化物检测的生物传感器。