Purpose

This paper aims to report on the development of a novel electrochemical amperometric immunosensor to diagnose early hepatocellular carcinoma (HCC) by detecting the Midkine (MDK) biomarker.

Design/methodology/approach

Anti-Midkine antibodies were immobilized covalently through carbodiimides chemistry on carbon screen-printed electrodes modified with carboxylated multi-walled carbon nanotubes. The development process was characterized using cyclic voltammetry, electrochemical impedimetric spectroscopy, Fourier transform infrared spectroscopy and atomic force microscopy. Differential pulse voltammetry was used to investigate the immunosensor performance in detecting MDK antigen within the concentration range of 1 pg/ml to 100 ng/ml.

Findings

MDK immunosensor exhibited high sensitivity and linearity with a detection limit of 0.8 pg/ml and a correlation coefficient of 0.99. The biosensor also demonstrated high selectivity, stability and reproducibility.

Originality/value

The developed MDK immunosensor could be a promising tool to diagnose HCC and reduce the number of related deaths.

中文翻译：

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开发用于早期诊断肝细胞癌的电化学免疫传感器

目的

本文旨在报告通过检测Midkine（MDK）生物标记物来诊断早期肝细胞癌（HCC）的新型电化学安培免疫传感器的开发。

设计/方法/方法

通过碳二亚胺化学作用将抗Midkine抗体共价固定在用羧基化多壁碳纳米管修饰的碳丝网印刷电极上。使用循环伏安法，电化学阻抗谱，傅里叶变换红外光谱和原子力显微镜对显影过程进行了表征。差动脉冲伏安法用于研究免疫传感器在1 pg / ml至100 ng / ml浓度范围内检测MDK抗原的性能。

发现

MDK免疫传感器具有高灵敏度和线性度，检出限为0.8 pg / ml，相关系数为0.99。该生物传感器还显示出高选择性，稳定性和可重复性。

创意/价值

开发的MDK免疫传感器可能是诊断HCC并减少相关死亡人数的有前途的工具。