ABSTRACT

The antagonistic activity of four Philippine EPN isolates of Steinernema abbasi Elawad et al. (SaMBLB) and Heterorhabditis indica Poinar et al., (HiPBCB, HiBSDS, and HiMAP) was investigated against Meloidogyne incognita (Kofoid & White) Chitwood. Fourteen days after inoculation, it was demonstrated that concomitant application of 1,000 live infective juveniles (IJ) of each EPN species and 200 M. incognita IJ significantly reduced root penetration consequently affecting juvenile development to the adult stage of M. incognita. The lowest number of M. incognita was found in plants treated with S. abbasi MBLB (1.90 ± 2.20) and H. indica PBCB (2.05 ± 2.61). Externally, formation of well-developed galls was only observed on tomato plants inoculated with RKN alone indicative of the negative effect of EPN to M. incognita root penetration. However, none of the EPN isolates had positive effect on the growth of M. incognita-treated tomato plants measured through plant height. This study provides the first insight on the biological control potential of these local EPN isolates for the management of M. incognita in the Philippines.

摘要

Steinernema abbasi Elawad 等人的四种菲律宾 EPN 分离株的拮抗活性。(SaMBLB) 和Heterorhabditis indica Poinar 等。,（HiPBCB、HiBSDS 和 HiMAP）针对南方根结线虫（Kofoid & White）Chitwood 进行了研究。接种后 14 天，证明同时施用 1,000 只 EPN 物种的活感染性幼虫 (IJ) 和 200 只南方根结线虫IJ 显着降低根部渗透，从而影响根部穿透到南方根结线虫的成虫阶段。的最低数目南方根结线虫与处理过的植物中发现S.阿巴斯MBLB（1.90±2.20）和籼稻PBCB (2.05 ± 2.61)。在外部，仅在单独接种 RKN 的番茄植株上观察到发育良好的瘿的形成，表明 EPN 对M. incognita根部渗透有负面影响。然而，没有一种 EPN 分离株对通过株高测量的南方根霉处理的番茄植株的生长产生积极影响。这项研究首次提供了关于这些本地 EPN 分离株在菲律宾管理南方根结线虫的生物控制潜力的见解。