Phycocyanin is a blue natural food colorant with multiple health benefits. Here we propose an efficient phycocyanin extraction method from Arthrospira platensis from Mexico. Three extraction methods were applied to optimize the extraction process, using water and buffer as solvents, with three pH values at two agitation times. The highest phycocyanin, 54.65 mg/g, was extracted from dry biomass with water as a solvent using an ultrasonication bar. The optimum condition of extraction was determined to be 1:50 biomass/solvent ratio for dry biomass, with the freeze/thaw method for 20 min repeated twice, and then agitated at 120 rpm for 24 h. The phycocyanin content was 48.88 mg/g biomass, with a purity of 0.47. For scalable phycocyanin productivity, the sonication method is recommended as there is no statistical difference. The phycocyanin stability was best at − 20 °C storage temperature at pH 7 for 35 days. Partial purification with ammonium sulfate was found to be suitable as a fractional precipitation method, first at 0–20% and then 20–65%, to get purity nearly 1. Total protein was found to be 55.52%, and total amino acids after phycocyanin extraction was 33%. The maximum phycocyanin yield using water as a solvent was the most interesting result regardless of the method used for extraction.

中文翻译：

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墨西哥Arthrospira platensis的藻蓝蛋白含量和营养成分：藻蓝蛋白的有效提取工艺和稳定性评估

藻蓝蛋白是一种具有多种健康益处的蓝色天然食用色素。在这里，我们提出了一种从墨西哥的Arthrospira platensis提取藻蓝蛋白的有效方法。用水和缓冲液为溶剂，采用两种萃取方法优化了萃取过程，在两次搅拌下均具有三个pH值。使用超声棒以水为溶剂从干燥的生物质中提取出最高的藻蓝蛋白，为54.65 mg / g。确定最佳提取条件为干燥生物质的生物质/溶剂比为1:50，重复20次冷冻/解冻方法20分钟，然后以120 rpm搅拌24 h。藻蓝蛋白含量为48.88 mg / g生物质，纯度为0.47。为了提高藻蓝蛋白的生产率，建议使用超声处理方法，因为没有统计学差异。藻蓝蛋白的稳定性在温度为− 20°C，pH为7的情况下保持35天最佳。发现使用硫酸铵进行部分纯化适合作为分步沉淀方法，首先以0–20％，然后以20–65％的纯度获得接近1的纯度。发现总蛋白为55.52％，藻蓝蛋白后的总氨基酸为提取率为33％。无论使用哪种提取方法，使用水作为溶剂的最大藻蓝蛋白产量是最令人感兴趣的结果。