ABSTRACT

Green synthesis of nanoparticles is one of the recent trends nowadays owing to its reduced toxic effects. Our study is aimed at evaluating the biological potential viz., antioxidant and anti-inflammatory properties of gold nanoparticles (AuNPs) biosynthesised using an aqueous extract of Commiphora wightii. The AuNPs synthesis was preliminarily confirmed by colour change from yellow to purple. Further, AuNPs synthesis was optimised at different ratio, pH and temperature. AuNPs were characterised using spectroscopic and microscopic techniques. FTIR analysis confirmed role of biomolecules in reduction and stabilisation of AuNPs. TEM analysis revealed polydispersed nanoparticles with an average size of 20.2 ± 6.6 nm. In vitroantioxidant activity of AuNPs were evaluated using DPPH, ABTS and H₂O₂ assays. The AuNPs showed significant radical scavenging ability. In vitro anti-inflammatory activity was studied for inhibition of albumin denaturation, Trypsin, LOX, and hyaluronidase enzymes. The AuNPs exhibited good activity as potent anti-inflammatory agents. Further in vivo studies are needed to explore them as good therapeutic agents.

摘要

纳米粒子的绿色合成是当今的最新趋势之一，因为它降低了毒性作用。我们的研究旨在评估使用Commiphora wightii水提取物生物合成的金纳米粒子 (AuNPs) 的生物潜力，即抗氧化和抗炎特性。通过颜色从黄色变为紫色，初步证实了 AuNPs 的合成。此外，AuNPs 的合成在不同的比例、pH 值和温度下进行了优化。使用光谱和显微技术对金纳米粒子进行了表征。FTIR 分析证实了生物分子在减少和稳定 AuNPs 中的作用。TEM 分析显示平均尺寸为 20.2 ± 6.6 nm 的多分散纳米粒子。体外_{使用 DPPH、ABTS 和 H 2} O ₂测定法评估 AuNPs 的抗氧化活性。AuNPs显示出显着的自由基清除能力。研究了体外抗炎活性以抑制白蛋白变性、胰蛋白酶、LOX 和透明质酸酶。AuNPs作为有效的抗炎剂表现出良好的活性。需要进一步的体内研究来探索它们作为良好的治疗剂。