In plants, endocytosis is essential for many developmental and physiological processes, including regulation of growth and development, hormone perception, nutrient uptake, and defense against pathogens. Our toolbox to modulate this process is, however, rather limited. Here, we report a conditional tool to impair endocytosis. We generated a partially functional TPLATE allele by substituting the most conserved domain of the TPLATE subunit of the endocytic TPLATE complex (TPC). This substitution destabilizes TPC and dampens the efficiency of endocytosis. Short-term heat treatment increases TPC destabilization and reversibly delocalizes TPLATE from the plasma membrane to aggregates in the cytoplasm. This blocks FM uptake and causes accumulation of various known endocytic cargoes at the plasma membrane. Short-term heat treatment therefore transforms the partially functional TPLATE allele into an effective conditional tool to impair endocytosis. Next to their role in endocytosis, several TPC subunits are also implicated in actin-regulated autophagosomal degradation. Inactivating TPC via the WDX mutation, however, does not impair autophagy, thus enabling specific and reversible modulation of endocytosis in planta.

在植物中，内吞作用对于许多发育和生理过程都是必不可少的，包括调节生长和发育，激素知觉，养分吸收以及对病原体的防御。但是，我们用来调整此过程的工具箱非常有限。在这里，我们报告一个条件工具，以损害内吞作用。我们通过取代内吞性TPLATE复合物（TPC）的TPLATE亚基的最保守结构域生成了部分功能性TPLATE等位基因。这种取代会破坏TPC的稳定性并削弱内吞作用的效率。短期热处理会增加TPC的去稳定作用，并使TPLATE从质膜可逆地脱离到细胞质中的聚集体。这会阻止FM的吸收，并导致各种已知的内吞货物在质膜上积聚。因此，短期热处理可将部分功能性TPLATE等位基因转变为损害内吞作用的有效条件工具。除了它们在胞吞作用中的作用外，一些TPC亚基也与肌动蛋白调节的自噬体降解有关。但是，通过WDX突变使TPC失活不会损害自噬，因此能够特异性和可逆地调节植物内吞作用。