Although the distribution of 8:2 polyfluoroalkyl phosphate diester (8:2 diPAP) in aquatic environments has been reported, details on its uptake, tissue specificity, and elimination in bivalve mollusks remain to be clarified. The present study is the first report on the accumulation and elimination of 8:2 diPAP in mussels (Mytilus galloprovincialis). The tissue-specific accumulation and depuration of 8:2 diPAP and its metabolites were investigated via semistatic seawater exposure (8:2 diPAP at a nominal concentration of 10 μg/L), through water-borne exposure with static daily renewal over a 72-h exposure period and a 360-h depuration period. The digestive gland was found to be the target organ where accumulation and biotransformation primarily occur. The bioaccumulation factor values (mL/g dry wt) in different organs were in the following order: digestive gland (1249) > adductor muscle (315) > gills (289) > gonad (82.9) > mantle (33.0). Moreover, the distribution of 8:2 diPAP among tissues may be related to the total protein content. The 8:2 diPAP tended to be excreted in feces. The compounds 8:2 fluorotelomer carboxylic acid, 8:2 fluorotelomer unsaturated carboxylic acid, 7:3 fluorotelomer carboxylic acid, perfluorooctanoic acid, and perfluoroheptanoic acid were detected and quantified as phase I metabolites, and the concentration of all phase I metabolites relative to the 8:2 diPAP concentration (72 h) was 0.304 mol%. A phase II metabolite, 8:2 fluorotelomer alcohol conjugated with sulfate, was detected but not quantitated in the digestive gland. A biotransformation pathway of 8:2 diPAP in M. galloprovincialis was proposed on the basis of the results obtained in the present study and previous studies. These findings improve our understanding of the accumulation of perfluorocarboxylic acids in bivalve mollusks. Environ Toxicol Chem 2021;40:1992–2004. © 2021 SETAC

中文翻译：

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8:2 多氟烷基磷酸二酯在 Mytilus galloprovincialis 中的吸收、组织分布和消除

尽管已经报道了 8:2 多氟烷基磷酸二酯 (8:2 diPAP) 在水生环境中的分布，但有关其在双壳贝类软体动物中的摄取、组织特异性和消除的详细信息仍有待澄清。本研究首次报道了贻贝（Mytilus galloprovincialis ）中 8:2 diPAP 的积累和消除。）。8:2 diPAP 及其代谢物的组织特异性积累和净化通过半静态海水暴露（标称浓度为 10 μg/L 的 8:2 diPAP），通过水传播暴露在 72- h 暴露期和 360 小时净化期。发现消化腺是主要发生积累和生物转化的靶器官。不同器官的生物蓄积因子值（mL/g干重）依次为：消化腺（1249）>内收肌（315）>鳃（289）>性腺（82.9）>外套膜（33.0）。此外，8:2 diPAP 在组织中的分布可能与总蛋白质含量有关。8:2 diPAP 倾向于从粪便中排出。化合物 8:2 含氟调聚物羧酸、8:2 含氟调聚物不饱和羧酸、7：3 氟调聚物羧酸、全氟辛酸和全氟庚酸作为 I 相代谢物被检测和量化，所有 I 相代谢物的浓度相对于 8:2 diPAP 浓度（72 小时）为 0.304 mol%。在消化腺中检测到一种 II 期代谢物，即与硫酸盐结合的 8:2 含氟调聚物醇，但未对其进行定量。8:2 diPAP 的生物转化途径M. galloprovincialis是根据本研究和以往研究中获得的结果提出的。这些发现提高了我们对双壳贝类中全氟羧酸积累的理解。 环境毒物化学2021；40:1992–2004。© 2021 SETAC