In this study, a sequencing batch reactor (SBR) with aerobic granular sludge (AGS) was operated with synthetic wastewater containing environmental relevant concentrations of 17β-estradiol (E2), 17α-ethinylestradiol (EE2) and sulfamethoxazole (SMX). Despite the presence of the studied PhAC, the granular fraction clearly predominated (TSS_gran/TSS ranging from 0.82 to 0.98) throughout the monitoring period, presenting aggregates with high organic fraction (VSS/TSS above 0.83) and good settling characteristics (SVI₅ ranging from 15 to 39 mL/gTSS). A principal component analysis (PCA) with quantitative image analysis (QIA) based data allowed to distinguish the different operational periods, namely with mature granules (CONT), and the E2, EE2, and SMX feeding periods. It further revealed a positive relationship between the biomass density, sludge settling ability, overall and granular biomass contents, granulation properties, granular biomass fraction and large granules fraction and size. Moreover, a discriminant analysis (DA) allowed to successfully discriminate not only the different operational periods, mainly by using the floccular apparent density, granular stratification and contents data, but also the PhAC presence in samples. The filamentous bacteria contents, sludge settling properties, settling properties stability and granular stratification, structure and contents parameters were found to be crucial for that purpose.

在这项研究中，带有好氧颗粒污泥（AGS）的测序间歇反应器（SBR）与含有环境相关浓度的17β-雌二醇（E2），17α-乙炔雌二醇（EE2）和磺胺甲恶唑（SMX）的合成废水一起运行。尽管研究PHAC的存在，粒状分数明确占优势（TSS_格兰/ TSS范围从0.82至0.98）在整个监测期间，具有很高的有机部分呈现聚集体（VSS / TSS以上0.83）和良好的沉降性能（SVI ₅范围从15到39 mL / g TSS）。主成分分析（PCA）和基于定量图像分析（QIA）的数据可以区分不同的操作周期，即成熟颗粒（CONT），E2，EE2和SMX进料周期。它进一步揭示了生物量密度，污泥沉降能力，总体和颗粒生物量含量，造粒特性，颗粒生物量级分和大颗粒级分和尺寸之间的正相关关系。此外，判别分析（DA）不仅可以成功地区分主要使用絮凝的表观密度，颗粒分层和含量数据，而且还可以分析样品中PhAC的存在，从而成功地区分不同的操作周期。丝状细菌含量，污泥沉降特性，沉降特性稳定性和颗粒分层，