Mycotoxins are secondary metabolites produced by various fungi and are known to have a significant negative impact on human and animal health. When feedstuffs are contaminated with mycotoxins, their toxicities may be caused a variety of diseases. In this study, the residual mycotoxins in feedstuffs were analyzed using LC–MS/MS incorporated with QuEChERS extraction. Analytical method validation was performed for LOD, LOQ, linearity, and recoveries with consideration of matrix effects prior to the residual analysis. They were all reached to the accepted range of validation level. Using 39 feedstuff samples (5 g) for mycotoxin analysis, nine samples were contaminated by four major mycotoxins such as fumonisin B1 (FB1), deoxynivalenol, fumonisin B2, and zearalenone. Among them, FB1 was detected at the highest concentration as 18.0943 mg/kg. The total sum of fumonisins in 39 samples did not exceed the maximum residual level (MRL) criterion set by Korean Food and Drug Administration. Altogether, intensive management of mycotoxins in Korean feedstuffs should be implemented with proper and routine monitoring, even their residual concentrations are not exceeded over the MRL levels because of high frequent detection found in this study.

中文翻译：

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用QuEChERS萃取法同时测定饲料中的13种霉菌毒素

霉菌毒素是各种真菌产生的次生代谢产物，已知对人类和动物健康具有重大的负面影响。当饲料中含有霉菌毒素污染时，其毒性可能引起多种疾病。在这项研究中，使用结合QuEChERS提取物的LC-MS / MS分析了饲料中残留的霉菌毒素。在残留分析之前，考虑到基质效应，对LOD，LOQ，线性和回收率进行了分析方法验证。他们都达到了认可水平的认可水平。使用39个饲料样品（5 g）进行霉菌毒素分析，有9个样品被四种主要霉菌毒素污染，如伏马毒素B1（FB1），脱氧雪茄烯醇，伏马毒素B2和玉米赤霉烯酮。其中，FB1的最高浓度为18.0943 mg / kg。39个样品中的伏马菌素总量不超过韩国食品药品监督管理局设定的最大残留水平（MRL）标准。总而言之，应该对韩国饲料中的霉菌毒素进行集约化管理，并进行适当的常规监测，即使由于本次研究中频繁发现，其残留浓度也不会超过最大残留限量（MRL）。