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Melatonin improves cognitive behavior, oxidative stress, and metabolism in tumor‐prone lethal giant larvae mutant of Drosophila melanogaster
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-04-05 , DOI: 10.1002/arch.21785 Kar‐Cheng Wong 1 , Srivani Sankaran 2 , Jaime J. Jayapalan 1, 3 , Perumal Subramanian 2 , Puteri S. Abdul‐Rahman 1, 3
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-04-05 , DOI: 10.1002/arch.21785 Kar‐Cheng Wong 1 , Srivani Sankaran 2 , Jaime J. Jayapalan 1, 3 , Perumal Subramanian 2 , Puteri S. Abdul‐Rahman 1, 3
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Mutant lethal giant larvae (lgl) flies (Drosophila melanogaster) are known to develop epithelial tumors with invasive characteristics. The present study has been conducted to investigate the influence of melatonin (0.025 mM) on behavioral responses of lgl mutant flies as well as on biochemical indices (redox homeostasis, carbohydrate and lipid metabolism, transaminases, and minerals) in hemolymph, and head and intestinal tissues. Behavioral abnormalities were quantitatively observed in lgl flies but were found normalized among melatonin‐treated lgl flies. Significantly decreased levels of lipid peroxidation products and antioxidants involved in redox homeostasis were observed in hemolymph and tissues of lgl flies, but had restored close to normalcy in melatonin‐treated flies. Carbohydrates including glucose, trehalose, and glycogen were decreased and increased in the hemolymph and tissues of lgl and melatonin‐treated lgl flies, respectively. Key enzymes of carbohydrate metabolism showed a significant increment in their levels in lgl mutants but had restored close to wild‐type baseline levels in melatonin‐treated flies. Variables of lipid metabolism showed significantly inverse levels in hemolymph and tissues of lgl flies, while normalization of most of these variables was observed in melatonin‐treated mutants. Lipase, chitinase, transaminases, and alkaline phosphatase showed an increment in their activities and minerals exhibited decrement in lgl flies; reversal of changes was observed under melatonin treatment. The impairment of cognition, disturbance of redox homeostasis and metabolic reprogramming in lgl flies, and restoration of normalcy in all these cellular and behavioral processes indicate that melatonin could act as oncostatic and cytoprotective agents in Drosophila.
中文翻译：
褪黑素可改善易患果蝇的致死性巨大幼虫突变体的认知行为，氧化应激和新陈代谢。
突变的致死性巨型幼虫（lgl）果蝇（果蝇Drosophila melanogaster）已知会发展出具有侵袭性特征的上皮肿瘤。进行本研究以研究褪黑激素（0.025 mM）对lgl突变果蝇行为反应以及血淋巴，头部和肠道中生化指标（氧化还原稳态，碳水化合物和脂质代谢，转氨酶和矿物质）的影响。组织。在lgl苍蝇中定量观察到行为异常，但发现在褪黑素治疗的lgl中行为异常已正常化苍蝇。在lgl蝇的血淋巴和组织中观察到参与氧化还原稳态的脂质过氧化产物和抗氧化剂水平显着降低，但在褪黑素处理过的蝇中恢复了接近正常水平。lgl和褪黑素处理过的lgl蝇的血淋巴和组织中的葡萄糖，海藻糖和糖原等碳水化合物分别减少和增加。碳水化合物代谢的关键酶在lgl突变体中显示出其水平的显着增加，但在褪黑素处理过的果蝇中已恢复至接近野生型基线水平。脂质代谢的变量显示lgl的血淋巴和组织中的逆水平显着升高苍蝇，而在褪黑素处理的突变体中观察到大多数这些变量的标准化。脂肪酶，几丁质酶，转氨酶和碱性磷酸酶的活性增加，而矿物质在lgl蝇中减少。在褪黑激素治疗下观察到变化的逆转。在所有这些细胞和行为过程中，lg1苍蝇的认知能力受损，氧化还原稳态的紊乱和代谢重编程以及正常状态的恢复都表明褪黑激素可以在果蝇中充当抑癌和细胞保护剂。
更新日期：2021-05-11
中文翻译：
褪黑素可改善易患果蝇的致死性巨大幼虫突变体的认知行为，氧化应激和新陈代谢。
突变的致死性巨型幼虫（lgl）果蝇（果蝇Drosophila melanogaster）已知会发展出具有侵袭性特征的上皮肿瘤。进行本研究以研究褪黑激素（0.025 mM）对lgl突变果蝇行为反应以及血淋巴，头部和肠道中生化指标（氧化还原稳态，碳水化合物和脂质代谢，转氨酶和矿物质）的影响。组织。在lgl苍蝇中定量观察到行为异常，但发现在褪黑素治疗的lgl中行为异常已正常化苍蝇。在lgl蝇的血淋巴和组织中观察到参与氧化还原稳态的脂质过氧化产物和抗氧化剂水平显着降低，但在褪黑素处理过的蝇中恢复了接近正常水平。lgl和褪黑素处理过的lgl蝇的血淋巴和组织中的葡萄糖，海藻糖和糖原等碳水化合物分别减少和增加。碳水化合物代谢的关键酶在lgl突变体中显示出其水平的显着增加，但在褪黑素处理过的果蝇中已恢复至接近野生型基线水平。脂质代谢的变量显示lgl的血淋巴和组织中的逆水平显着升高苍蝇，而在褪黑素处理的突变体中观察到大多数这些变量的标准化。脂肪酶，几丁质酶，转氨酶和碱性磷酸酶的活性增加，而矿物质在lgl蝇中减少。在褪黑激素治疗下观察到变化的逆转。在所有这些细胞和行为过程中，lg1苍蝇的认知能力受损，氧化还原稳态的紊乱和代谢重编程以及正常状态的恢复都表明褪黑激素可以在果蝇中充当抑癌和细胞保护剂。
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