SummaryThe present study was undertaken to analyze the relative abundance (RA) of pluripotency-associated genes (NANOG, OCT4, SOX2, c-MYC, and FOXD3) in different grades of immature oocytes and various stages of in vitro-produced buffalo embryos using RT-qPCR. Results showed that the RA of NANOG, OCT4, and FOXD3 transcripts was significantly higher (P < 0.05) in A grade oocytes compared with the other grades of oocytes. The RA of the c-MYC transcript was significantly higher (P < 0.05) in A grade compared with the C and D grades of oocytes, but the values did not differ significantly from the B grade of oocytes. The RA of the SOX2 transcript was almost similar in all grades of the oocytes. The expression levels of NANOG (P > 0.05), OCT4 (P > 0.05), c-MYC (P > 0.05) and SOX2 (P < 0.05) were higher in the blastocysts compared with the other stages of the embryos. Markedly, FOXD3 expression was significantly higher (P < 0.05) in 8–16-cell embryos compared with the 2-cell and 4-cell embryos and blastocyst, but did not differ significantly from the morula stage of the embryos. In the study, the majority of pluripotency-associated genes showed higher expression in A grade immature oocytes. Therefore, it is concluded that the A grade oocytes appeared to be more developmental competent and are suitable candidates for nuclear cloning research in buffalo. In buffalo, NANOG, OCT4, SOX2, and c-MYC are highly expressed in blastocysts compared with the other stages of embryos.

中文翻译：

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未成熟卵母细胞和体外生产的水牛胚胎（Bubalus bubalis）中多能性相关候选基因的相对丰度

总结本研究旨在分析多能性相关基因的相对丰度 (RA) (纳米油,10月4日,SOX2， C-我的C， 和福克斯D3) 在不同等级的未成熟卵母细胞和不同阶段体外- 使用 RT-qPCR 生产水牛胚胎。结果表明，RA纳米油,10月4日， 和福克斯D3成绩单明显更高（磷< 0.05) 与其他等级的卵母细胞相比，A 级卵母细胞。c的RA-我的C成绩单明显更高（磷< 0.05) 与卵母细胞的 C 和 D 级相比，A 级的值与卵母细胞的 B 级没有显着差异。RA的SOX2在所有级别的卵母细胞中，转录物几乎相似。的表达水平纳米油(磷> 0.05),10月4日(磷> 0.05), c-我的C(磷> 0.05) 和SOX2(磷< 0.05) 与胚胎的其他阶段相比，胚泡中的含量更高。明显地，福克斯D3表达明显更高（磷< 0.05）在 8-16 细胞胚胎中与 2 细胞和 4 细胞胚胎和囊胚相比，但与胚胎的桑椹胚阶段没有显着差异。在该研究中，大多数多能性相关基因在 A 级未成熟卵母细胞中表现出较高的表达。因此，得出的结论是，A 级卵母细胞似乎更具发育能力，是水牛核克隆研究的合适候选者。在水牛城，纳米油,10月4日,SOX2, 和 c-我的C与胚胎的其他阶段相比，在囊胚中高度表达。