Among the various bacterial pathogens associated with the aquaculture environment, Vibrio parahaemolyticus the important one from shrimp and human health aspects. Though having been around for several decades, phage-based control of bacterial pathogens (phage therapy) has recently re-emerged as an attractive alternative due to the availability of modern phage characterization tools and the global emergence of antibiotic-resistant bacteria. In the present study, a total of 12 V. parahaemolyticus specific phages were isolated from 264 water samples collected from inland saline shrimp culture farms. During the host range analysis against standard/field isolates of V. parahaemolyticus and other bacterial species, lytic activity was observed against 2.3–45.5% of tested V. parahaemolyticus isolates. No lytic activity was observed against other bacterial species. For genomic characterization, high-quality phage nucleic acid with concentrations ranging from 7.66 to 220 ng/µl was isolated from 9 phages. After digestion treatments with DNase, RNase and S1 nuclease, the nature of phage nucleic acid was determined as ssDNA and dsDNA for 7 and 2 phages respectively. During transmission electron microscopy analysis of phage V5, it was found to have a filamentous shape making it a member of the family Inoviridae. During efficacy study of phage against V. parahaemolyticus in shrimp, 78.1% reduction in bacterial counts was observed within 1 h of phage application. These results indicate the potential of phage therapy for the control of V. parahaemoyticus in shrimp.

在与水产养殖环境相关的各种细菌病原体中，副溶血性弧菌是对虾和人类健康方面的重要病原体。尽管已经存在了几十年，但由于现代噬菌体表征工具的可用性和抗生素抗性细菌的全球出现，基于噬菌体的细菌病原体控制（噬菌体疗法）最近重新成为一种有吸引力的替代方案。在本研究中， 从内陆盐渍对虾养殖场采集的 264 个水样中，共分离出12 个副溶血性弧菌特异性噬菌体。在针对副溶血性弧菌的标准/现场分离物的宿主范围分析期间和其他细菌物种，对 2.3-45.5% 的受试副溶血性弧菌分离株观察到溶解活性。没有观察到针对其他细菌种类的裂解活性。对于基因组表征，从 9 个噬菌体中分离出浓度范围为 7.66 至 220 ng/µl 的高质量噬菌体核酸。在用 DNase、RNase 和 S1 核酸酶消化处理后，噬菌体核酸的性质分别确定为 7 个和 2 个噬菌体的 ssDNA 和 dsDNA。在对噬菌体 V5 进行透射电子显微镜分析期间，发现它具有丝状形状，使其成为Inoviridae家族的成员。在噬菌体对副溶血性弧菌的功效研究中在虾中，在应用噬菌体 1 小时内观察到细菌数量减少了 78.1%。这些结果表明噬菌体疗法在控制虾中的副嗜血弧菌方面具有潜力。