Purpose

To evaluate the levels and regulation of tear film inflammatory proteins in contact lens-related dry eye (CLDE).

Methods

One hundred healthy, daily wear (non-overnight), experienced soft contact lens wearers were classified into normal (n = 50) and CLDE (n = 50) groups based on Contact Lens and Dry Eye Questionnaire scores, tear break-up times, and comfort (a two-hour difference between total and comfortable daily lens wear hours). Tear samples (up to 5 μL) were collected by capillary extraction from the inferior meniscus of each eye, and pooled tear samples (10 per group) were tested using a customized Quantibody array. Mann Whitney tests with the Benjamini-Hochberg procedure with a 5% false discovery rate were used to compare the normal and CLDE groups.

Results

Relative to the normal group, the CLDE group showed a significantly increased tear concentration of several inflammatory mediators, including interleukin (IL)-7 (p = 0.001), IL-8 (p = 0.001), IL-13 (p = 0.001), IL-15 (p = 0.001), IL-12 p70 (p = 0.002), growth-related oncogene-alpha/ chemokine (CXC motif) ligand 1 (p = 0.003), granulocyte–colony stimulating factor (p = 0.005), IL-11 (p = 0.008), epidermal growth factor receptor (p = 0.01), IL-1 receptor antagonist (RA) (p = 0.013), macrophage colony-stimulating factor (p = 0.013), Eotaxin/CC motif chemokine ligand 11 (CCL11) (p = 0.016), and IL-2 (p = 0.016). The following cytokines were increased three-fold or more in the CLDE group: IL-13 (p = 0.001), Eotaxin/CCL11 (p = 0.016), and IL-1RA (p = 0.013).

Conclusions

Several inflammatory markers, including interleukins, were increased in tears of subjects with CLDE. These results support a growing body of evidence that suggests a potential role of inflammation in CLDE.

中文翻译：

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与隐形眼镜相关的干眼症相关的炎症蛋白

目的

评估隐形眼镜相关干眼症 (CLDE) 中泪膜炎症蛋白的水平和调节。

方法

根据隐形眼镜和干眼问卷评分、泪液破裂时间、和舒适度（总和舒适的每日镜片佩戴时间相差两个小时）。通过毛细血管从每只眼睛的下半月板提取泪液样本（最多 5 μL），并使用定制的 Quantibody 阵列测试合并的泪液样本（每组 10 个）。使用 Benjamini-Hochberg 程序的 Mann Whitney 检验以 5% 的错误发现率来比较正常组和 CLDE 组。

结果

相对于正常组，CLDE 组的几种炎症介质的泪液浓度显着增加，包括白细胞介素 (IL)-7 (p = 0.001)、IL-8 (p = 0.001)、IL-13 (p = 0.001) , IL-15 (p = 0.001), IL-12 p70 (p = 0.002), 生长相关癌基因-α/趋化因子 (C X C 基序) 配体 1 (p = 0.003), 粒细胞集落刺激因子 (p = 0.005)、IL-11 (p = 0.008)、表皮生长因子受体 (p = 0.01)、IL-1 受体拮抗剂 (RA) (p = 0.013)、巨噬细胞集落刺激因子 (p = 0.013)、Eotaxin/C C 基序趋化因子配体 11 (CCL11) (p = 0.016) 和 IL-2 (p = 0.016)。CLDE 组中以下细胞因子增加了三倍或更多：IL-13 (p = 0.001)、Eotaxin/CCL11 (p = 0.016) 和 IL-1RA (p = 0.013)。

结论

包括白细胞介素在内的几种炎症标志物在患有 CLDE 的受试者的眼泪中增加。这些结果支持越来越多的证据表明炎症在 CLDE 中的潜在作用。