Two antimalaria alkaloids, febrifugine and isofebrifugine, were successfully separated from total alkaloids of Dichroa febrifuga roots by one-step preparative countercurrent chromatography with a selected biphasic solvent system. The selected biphasic solvent system was composed of chloroform: methanol: water (2:1:1, v/v) according to partition performance of the two target components. Selection of biphasic solvent system was conducted by high performance liquid chromatography combined with high performance thin layer chromatography, which greatly assisted the screening procedure for biphasic solvent system. Totally, 50 mg of total alkaloid was separated by one-step preparative countercurrent chromatography, yielding 12 mg of febrifugine and 9 mg of isofebrifugine with more than 98.0% purity, respectively.

中文翻译：

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逆流色谱法制备分离二色菊根中两种同分异构抗疟生物碱热药和异热药

两个抗疟疾生物碱，常山碱和异常山碱，已成功地从总生物碱分离常山根通过一步法制备逆流色谱与选定的两相溶剂体系。根据两种目标组分的分配性能，选定的双相溶剂系统由氯仿:甲醇:水 (2:1:1, v/v) 组成。双相溶剂体系的筛选采用高效液相色谱结合高效薄层色谱法进行，极大地辅助了双相溶剂体系的筛选过程。通过一步制备型逆流色谱法，总共分离出50mg总生物碱，分别得到12mg的非本药和9mg的异热药，纯度超过98.0%。