Literature data about semi-volatile organic compounds in plants and the effect of cerium oxide nanoparticles on them are scarce. Surface modification of nanoparticles may change nanoparticle-environment interaction, and therefore affects compounds in plants. In this research, uncoated and glucose-, levan-, and pullulan-coated cerium oxide nanoparticles were used for wheat and pea treatment during the growth. The aim was the screening of semi-volatile organic compounds from plants’ shoots using comprehensive two-dimensional gas chromatography–mass spectrometry, a powerful separation technique allowing to reach unique separation resolution, and investigation of qualitative changes after the treatment with coated cerium oxide nanoparticles. The results were analyzed by the identification of individual peaks and fingerprint analysis by image processing. Wheat samples contained a higher number of semi-volatile organic compounds (108) compared to pea (77) but were less affected by the treatments with coated nanoparticles. The highest number of compounds was detected in wheat after the treatment with levan- and pullulan-coated nanoparticles, and in pea after treatment with levan-coated nanoparticles. This article reports a successful application of a semi-volatile organic compounds profile presented only as categorical variables and unique fingerprint images for the inter-cultivar recognition. This method may be useful in screening nanoparticles’ effects on different plants.

中文翻译：

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综合二维气相色谱法筛选经纳米氧化铈处理的植物中的半挥发性化合物


关于植物中半挥发性有机化合物以及氧化铈纳米颗粒对其影响的文献数据很少。纳米颗粒的表面修饰可能会改变纳米颗粒与环境的相互作用，从而影响植物中的化合物。在这项研究中，未涂层和葡萄糖、果聚糖和普鲁兰多糖涂层的氧化铈纳米颗粒用于小麦和豌豆生长过程中的处理。目的是使用综合二维气相色谱-质谱法筛选植物芽中的半挥发性有机化合物，这是一种强大的分离技术，可以达到独特的分离分辨率，并研究用涂覆的氧化铈纳米粒子处理后的质变。通过图像处理识别各个峰和进行指纹分析来分析结果。与豌豆（77）相比，小麦样品含有更多数量的半挥发性有机化合物（108），但受涂层纳米粒子处理的影响较小。在用果聚糖和支链淀粉涂覆的纳米颗粒处理后的小麦中检测到的化合物数量最多，在用果聚糖涂覆的纳米颗粒处理后的豌豆中检测到的化合物数量最多。本文报道了仅作为分类变量和独特指纹图像呈现的半挥发性有机化合物谱的成功应用，用于品种间识别。该方法可用于筛选纳米颗粒对不同植物的影响。