Design of experiments (DoE) is a valuable tool for the optimization of quantitative bioanalytical methods utilizing liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). Liquid chromatography mass spectrometry (LC–MS) is composed of several processes, including, liquid introduction and analyte ionization. The goal is to transfer analytes from atmospheric pressure to vacuum and maintain conditions that are compatible for both LC and MS. These processes involve many experimental factors which need to be simultaneously optimized to obtain maximum sensitivity and resolution at minimum retention time. In this tutorial, the basic concepts of DoE will be explained with focus on practical use of DoE. Three case studies optimized with DoE for liquid chromatography tandem mass spectrometry (LC–MS/MS) quantitative assays will then be presented.

中文翻译：

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液相色谱与串联质谱生物分析联用的开发和优化实验设计


实验设计 (DoE) 是利用液相色谱与串联质谱 (LC-MS/MS) 联用来优化定量生物分析方法的宝贵工具。液相色谱质谱法 (LC-MS) 由多个过程组成，包括液体引入和分析物电离。目标是将分析物从大气压转移到真空，并维持适合 LC 和 MS 的条件。这些过程涉及许多实验因素，需要同时优化这些因素，以便在最短的保留时间内获得最大的灵敏度和分辨率。在本教程中，将解释 DoE 的基本概念，重点是 DoE 的实际使用。随后将介绍三个利用 DoE 优化的液相色谱串联质谱 (LC-MS/MS) 定量分析案例研究。